Protein Domain : IPR004540

Type:  Family Name:  Translation elongation factor EFG/EF2
Description:  Translation elongation factors are responsible for two main processes during protein synthesis on the ribosome [, , ]. EF1A (or EF-Tu) is responsible for the selection and binding of the cognate aminoacyl-tRNA to the A-site (acceptor site) of the ribosome. EF2 (or EF-G) is responsible for the translocation of the peptidyl-tRNA from the A-site to the P-site (peptidyl-tRNA site) of the ribosome, thereby freeing the A-site for the next aminoacyl-tRNA to bind. Elongation factors are responsible for achieving accuracy of translation and both EF1A and EF2 are remarkably conserved throughout evolution.EF-G is a large, five-domain GTPase that promotes the directional movement of mRNA and tRNAs on the ribosome in a GTP-dependent manner. Unlike other GTPases, but by analogy to the myosin motor, EF-G performs its function of powering translocation in the GDP-bound form; that is, in a kinetically stable ribosome-EF-G(GDP) complex formed by GTP hydrolysis on the ribosome. The complex undergoes an extensive structural rearrangement, in particular affecting the small ribosomal subunit, which leads to mRNA-tRNA movement. Domain 4, which extends from the 'body' of the EF-G molecule much like a lever arm, appears to be essential for the structural transition to take place. In a hypothetical model, GTP hydrolysis induces a conformational change in the G domain of EF-G, which affects the interactions with neighbouring domains within EF-G. The resulting rearrangement of the domains relative to each other generates conformational strain in the ribosome to which EF-G is fixed. Because of structural features of the tRNA-ribosome complex, this conformational strain results in directional tRNA-mRNA movement. The functional parallels between EF-G and motor proteins suggest that EF-G differs from classical G-proteins in that it functions as a force-generating mechanochemical device rather than a conformational switch [].Every completed bacterial genome has at least one copy, but some species have additional EF-G-like proteins. The closest homologue to canonical (e.g. Escherichia coli) EF-G in the spirochetes clusters as if it is derived from mitochondrial forms, while a more distant second copy is also present. Synechocystis sp.(strain PCC 6803) has a few proteins more closely related to EF-G than to any other characterised protein. Two of these resemble E. coli EF-G more closely than does the best match from the spirochetes; it may be that both function as authentic EF-G. Short Name:  Transl_elong_EFG/EF2
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1 Child Features

DB identifier Type Name
IPR030848 Family Elongation factor G, chloroplastic

8 Contains

DB identifier Type Name
IPR020568 Domain Ribosomal protein S5 domain 2-type fold
IPR009022 Domain Elongation factor G, III-V domain
IPR000640 Domain Translation elongation factor EFG, V domain
IPR004161 Domain Translation elongation factor EFTu/EF1A, domain 2
IPR000795 Domain Transcription factor, GTP-binding domain
IPR009000 Domain Translation protein, beta-barrel domain
IPR005517 Domain Translation elongation factor EFG/EF2, domain IV
IPR014721 Domain Ribosomal protein S5 domain 2-type fold, subgroup

2 Cross Referencess

Identifier
TIGR00484
MF_00054_B

0 Found In

4 GO Annotations

GO Term Gene Name
GO:0003746 IPR004540
GO:0005525 IPR004540
GO:0006414 IPR004540
GO:0005622 IPR004540

4 Ontology Annotations

GO Term Gene Name
GO:0003746 IPR004540
GO:0005525 IPR004540
GO:0006414 IPR004540
GO:0005622 IPR004540

0 Parent Features

798 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
141453 D8QWC2 PAC:15409675 Selaginella moellendorffii 694  
143627 D8R4J4 PAC:15417020 Selaginella moellendorffii 683  
evm.model.supercontig_29.106 PAC:16416129 Carica papaya 787  
29983.m003175 B9RVY0 PAC:16814943 Ricinus communis 699  
30190.m010996 B9RBP7 PAC:16823358 Ricinus communis 789  
Cucsa.121140.4 PAC:16961280 Cucumis sativus 630  
Cucsa.121140.1 PAC:16961277 Cucumis sativus 753  
Cucsa.121140.3 PAC:16961279 Cucumis sativus 723  
Cucsa.121140.2 PAC:16961278 Cucumis sativus 753  
Cucsa.302140.1 PAC:16975719 Cucumis sativus 777  
orange1.1g004311m A0A067H188 PAC:18137110 Citrus sinensis 762  
orange1.1g004649m A0A067HCU4 PAC:18137112 Citrus sinensis 740  
orange1.1g004316m A0A067H3W7 PAC:18137111 Citrus sinensis 761  
orange1.1g004615m A0A067EAZ8 PAC:18110588 Citrus sinensis 742  
orange1.1g007325m A0A067E3F9 PAC:18110590 Citrus sinensis 608  
orange1.1g008294m A0A067EBU7 PAC:18110591 Citrus sinensis 571  
orange1.1g004038m A0A067DZX8 PAC:18110587 Citrus sinensis 777  
orange1.1g005154m A0A067DZJ3 PAC:18110589 Citrus sinensis 711  
AT1G45332.1 Q9C641 PAC:19651928 Arabidopsis thaliana 754  
AT1G62750.1 Q9SI75 PAC:19649709 Arabidopsis thaliana 783  
AT2G45030.1 F4IW10 PAC:19641120 Arabidopsis thaliana 754  
Thhalv10001325m V4N1Y1 PAC:20188864 Eutrema salsugineum 753  
Thhalv10023290m V4KP43 PAC:20201349 Eutrema salsugineum 783  
Ciclev10019139m V4T0I5 PAC:20807330 Citrus clementina 684  
Ciclev10018943m V4VLJ4 PAC:20807329 Citrus clementina 777  
Ciclev10019198m V4TYB7 PAC:20811715 Citrus clementina 663  
Ciclev10018974m V4U8R6 PAC:20811714 Citrus clementina 761  
Lus10004326 PAC:23161727 Linum usitatissimum 781  
Lus10018604 PAC:23180469 Linum usitatissimum 759  
Lus10039840 PAC:23165010 Linum usitatissimum 739  

4 Publications

First Author Title Year Journal Volume Pages PubMed ID
            12471894
            12932732
            15922593
            12762045