Protein Domain : IPR007627

Type:  Domain Name:  RNA polymerase sigma-70 region 2
Description:  The bacterial core RNA polymerase complex, which consists of five subunits, is sufficient for transcription elongation and termination but is unable to initiate transcription. Transcription initiation from promoter elements requires a sixth, dissociable subunit called a sigma factor, which reversibly associates with the core RNA polymerase complex to form a holoenzyme []. RNA polymerase recruits alternative sigma factors as a means of switching on specific regulons. Most bacteria express a multiplicity of sigma factors. Two of these factors, sigma-70 (gene rpoD), generally known as the major or primary sigma factor, and sigma-54 (gene rpoN or ntrA) direct the transcription of a wide variety of genes. The other sigma factors, known as alternative sigma factors, are required for the transcription of specific subsets of genes.With regard to sequence similarity, sigma factors can be grouped into two classes, the sigma-54 and sigma-70 families. Sequence alignments of the sigma70 family members reveal four conserved regions that can be further divided into subregions eg. sub-region 2.2, which may be involved in the binding of the sigma factor to the core RNA polymerase; and sub-region 4.2, which seems to harbor a DNA-binding 'helix-turn-helix' motif involved in binding the conserved -35 region of promoters recognised by the major sigma factors [, ]. Region 2 of sigma-70 is the most conserved region of the entire protein. All members of this class of sigma-factor contain region 2. The high conservation is due to region 2 containing both the -10 promoter recognition helix and the primary core RNA polymerase binding determinant. The core-binding helix, interacts with the clamp domain of the largest polymerase subunit, beta prime [, ]. The aromatic residues of the recognition helix, found at the C terminus of this domain are thought to mediate strand separation, thereby allowing transcription initiation [, ]. Short Name:  RNA_pol_sigma70_r2
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0 Child Features

1 Contains

DB identifier Type Name
IPR000838 Conserved_site RNA polymerase sigma factor 70, ECF, conserved site

1 Cross References

Identifier
PF04542

32 Found Ins

DB identifier Type Name
IPR014284 Domain RNA polymerase sigma-70 like domain
IPR013325 Domain RNA polymerase sigma factor, region 2
IPR000943 Domain RNA polymerase sigma-70
IPR016262 Family RNA polymerase sigma factor, SigB/C/D/F, plastid
IPR012759 Family RNA polymerase sigma factor RpoH, proteobacteria
IPR012760 Domain RNA polymerase sigma factor RpoD, C-terminal
IPR016263 Family RNA polymerase sporulation-specific sigma factor
IPR017848 Family RNA polymerase sigma factor, RpoD-like, cyanobacteria
IPR014209 Family RNA polymerase sigma-K type
IPR012761 Family RNA polymerase sigma factor RpoS
IPR016371 Family RNA polymerase sigma-H factor
IPR014218 Family RNA polymerase sigma-H type
IPR014200 Family RNA polymerase sigma-E type
IPR012845 Family RNA polymerase sigma factor, FliA/WhiG
IPR014305 Family RNA polymerase sigma-G type, actinobacteria
IPR014327 Family RNA polymerase sigma-70, Bacteroidetes type
IPR014325 Family RNA polymerase sigma-E factor, actinobacteria
IPR014326 Family RNA polymerase sigma-70 factor, Planctomycetaceae
IPR014322 Family RNA polymerase sigma-B/F/G type
IPR014236 Family RNA polymerase sigma-F type
IPR014288 Family RNA polymerase sigma-B factor
IPR014212 Family RNA polymerase sigma-G type
IPR014331 Family RNA polymerase sigma-70 ECF-like, Rhodopirellula baltica
IPR014303 Family RNA polymerase sigma-70, ECF type
IPR014304 Family RNA polymerase sigma-Z type
IPR014300 Family RNA polymerase sigma-V type
IPR014244 Family RNA polymerase sigma-I type
IPR014286 Family RNA polymerase sigma-70 RpoE type
IPR014293 Family RNA polymerase sigma-70, actinobacteria
IPR014289 Family RNA polymerase sigma-24-related

5 GO Annotations

GO Term Gene Name
GO:0003677 IPR007627
GO:0003700 IPR007627
GO:0016987 IPR007627
GO:0006352 IPR007627
GO:0006355 IPR007627

5 Ontology Annotations

GO Term Gene Name
GO:0003677 IPR007627
GO:0003700 IPR007627
GO:0016987 IPR007627
GO:0006352 IPR007627
GO:0006355 IPR007627

0 Parent Features

3256 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
147096 D8RGX1 PAC:15401952 Selaginella moellendorffii 458  
53596 D8S3G8 PAC:15415781 Selaginella moellendorffii 326  
117159 D8SHL6 PAC:15403291 Selaginella moellendorffii 292  
266972 D8R5F3 PAC:15412233 Selaginella moellendorffii 249  
evm.model.supercontig_125.39 PAC:16407269 Carica papaya 443  
evm.model.supercontig_3.54 PAC:16416838 Carica papaya 547  
evm.model.supercontig_3807.1 PAC:16418945 Carica papaya 333  
evm.model.supercontig_56.16 PAC:16422721 Carica papaya 564  
evm.model.supercontig_73.29 PAC:16425606 Carica papaya 357  
29794.m003368 B9RRX0 PAC:16808728 Ricinus communis 560  
29864.m001444 B9RVK0 PAC:16811756 Ricinus communis 389  
30078.m002307 B9S0J0 PAC:16817418 Ricinus communis 548  
30148.m001412 B9SDA3 PAC:16820557 Ricinus communis 569  
30148.m001468 B9SDF8 PAC:16820612 Ricinus communis 508  
30169.m006532 B9RIL8 PAC:16821332 Ricinus communis 501  
30445.m000022 B9THE0 PAC:16824427 Ricinus communis 315  
30892.m000027 B9TKG6 PAC:16824679 Ricinus communis 190  
32775.m000024 B9TEC6 PAC:16825552 Ricinus communis 271  
33001.m000032 PAC:16825645 Ricinus communis 122  
34613.m000038 B9TJU4 PAC:16826284 Ricinus communis 139  
43220.m000021 B9TQM5 PAC:16827580 Ricinus communis 122  
47514.m000023 B9TMI4 PAC:16828001 Ricinus communis 172  
47961.m000024 PAC:16828045 Ricinus communis 141  
28920.m000046 PAC:16801985 Ricinus communis 169  
Cucsa.362600.1 A0A0A0K917 PAC:16980950 Cucumis sativus 580  
Cucsa.012950.1 PAC:16951584 Cucumis sativus 585  
Cucsa.012950.2 A0A0A0LCQ9 PAC:16951585 Cucumis sativus 547  
Cucsa.124610.1 A0A0A0KEQ6 PAC:16961743 Cucumis sativus 562  
Cucsa.073340.2 PAC:16955602 Cucumis sativus 473  
Cucsa.073340.1 A0A0A0LQR2 PAC:16955601 Cucumis sativus 508  

5 Publications

First Author Title Year Journal Volume Pages PubMed ID
            3052291
            1597408
            3092189
            11931761
            8858155