Protein Domain : IPR012754

Type:  Family Name:  DNA-directed RNA polymerase, subunit beta-prime
Description:  DNA-directed RNA polymerases (also known as DNA-dependent RNA polymerases) are responsible for the polymerisation of ribonucleotides into a sequence complementary to the template DNA. In eukaryotes, there are three different forms of DNA-directed RNA polymerases transcribing different sets of genes. Most RNA polymerases are multimeric enzymes and are composed of a variable number of subunits. The core RNA polymerase complex consists of five subunits (two alpha, one beta, one beta-prime and one omega) and is sufficient for transcription elongation and termination but is unable to initiate transcription. Transcription initiation from promoter elements requires a sixth, dissociable subunit called a sigma factor, which reversibly associates with the core RNA polymerase complex to form a holoenzyme []. The core RNA polymerase complex forms a "crab claw"-like structure with an internal channel running along the full length []. The key functional sites of the enzyme, as defined by mutational and cross-linking analysis, are located on the inner wall of this channel.RNA synthesis follows after the attachment of RNA polymerase to a specific site, the promoter, on the template DNA strand. The RNA synthesis process continues until a termination sequence is reached. The RNA product, which is synthesised in the 5' to 3'direction, is known as the primary transcript. Eukaryotic nuclei contain three distinct types of RNA polymerases that differ in the RNA they synthesise:RNA polymerase I: located in the nucleoli, synthesises precursors of most ribosomal RNAs.RNA polymerase II: occurs in the nucleoplasm, synthesises mRNA precursors. RNA polymerase III: also occurs in the nucleoplasm, synthesises the precursors of 5S ribosomal RNA, the tRNAs, and a variety of other small nuclear and cytosolic RNAs. Eukaryotic cells are also known to contain separate mitochondrial and chloroplast RNA polymerases. Eukaryotic RNA polymerases, whose molecular masses vary in size from 500 to 700 kDa, contain two non-identical large (>100 kDa) subunits and an array of up to 12 different small (less than 50 kDa) subunits.This entry represents the beta-prime subunit, RpoC, found in most bacteria. It excludes some, mainly cyanobacterial, species where RpoC is replaced by two homologous proteins that include an additional domain. One arm of the "claw" is predominantly formed by this subunit, the other being predominantly formed by the beta subunit. The active site of the enzyme is defined by three invariant aspartate residues within the beta-prime subunit []. Short Name:  DNA-dir_RpoC_beta_prime
Paste the following link

0 Child Features

6 Contains

DB identifier Type Name
IPR007083 Domain RNA polymerase Rpb1, domain 4
IPR007066 Domain RNA polymerase Rpb1, domain 3
IPR007081 Domain RNA polymerase Rpb1, domain 5
IPR000722 Domain RNA polymerase, alpha subunit
IPR006592 Domain RNA polymerase, N-terminal
IPR007080 Domain RNA polymerase Rpb1, domain 1

2 Cross Referencess

Identifier
TIGR02386
MF_01322

0 Found In

3 GO Annotations

GO Term Gene Name
GO:0003677 IPR012754
GO:0003899 IPR012754
GO:0006351 IPR012754

3 Ontology Annotations

GO Term Gene Name
GO:0003677 IPR012754
GO:0003899 IPR012754
GO:0006351 IPR012754

0 Parent Features

4 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
Araha.11756s0289.1.p PAC:28856480 Arabidopsis halleri 1382  
Brdisv1pangenome1007585m.p PAC:33656559 Brachypodium distachyon Pangenome 1286  
Brdisv1BdTR8i1044374m.p PAC:34241897 Brachypodium distachyon BdTR8i 1236  
Brdisv1BdTR11A1042575m.p PAC:35691374 Brachypodium distachyon BdTR11a 1286  

3 Publications

First Author Title Year Journal Volume Pages PubMed ID
            3052291
            10499798
            12000971