Protein Domain : IPR004527

Type:  Family Name:  Glutamate-tRNA ligase, bacterial/mitochondrial
Description:  Glutamate-tRNA ligase (also known as glutamyl-tRNA synthetase)() is a class Ic ligase and shows several similarities with glutamate-tRNA ligase concerning structure and catalytic properties. It is an alpha2 dimer. To date one crystal structure of a glutamate-tRNA ligase (Thermus thermophilus) has been solved. The molecule has the form of a bent cylinder and consists of four domains. The N-terminal half (domains 1 and 2) contains the 'Rossman fold' typical for class I ligases and resembles the corresponding part of Escherichia coliGlnRS, whereas the C-terminal half exhibits a GluRS-specific structure [].The aminoacyl-tRNA synthetase (also known as aminoacyl-tRNA ligase) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction. These proteins differ widely in size and oligomeric state, and have limited sequence homology []. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric []. Class II aminoacyl-tRNA synthetases share an anti-parallel beta-sheet fold flanked by alpha-helices [], and are mostly dimeric or multimeric, containing at least three conserved regions [, , ]. However, tRNA binding involves an alpha-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan and valine belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, lysine, phenylalanine, proline, serine, and threonine belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c. Short Name:  Glu-tRNA-ligase_bac/mito
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0 Child Features

7 Contains

DB identifier Type Name
IPR014729 Domain Rossmann-like alpha/beta/alpha sandwich fold
IPR020751 Domain Aminoacyl-tRNA synthetase, class I, anticodon-binding domain, subdomain 2
IPR020058 Domain Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain
IPR008925 Domain Aminoacyl-tRNA synthetase, class I, anticodon-binding
IPR020752 Domain Aminoacyl-tRNA synthetase, class I, anticodon-binding domain, subdomain 1
IPR020061 Domain Glutamyl/glutaminyl-tRNA synthetase, class Ib, alpha-bundle domain
IPR001412 Conserved_site Aminoacyl-tRNA synthetase, class I, conserved site

2 Cross Referencess

Identifier
TIGR00464
MF_00022_B

0 Found In

5 GO Annotations

GO Term Gene Name
GO:0000166 IPR004527
GO:0004818 IPR004527
GO:0005524 IPR004527
GO:0006424 IPR004527
GO:0005737 IPR004527

5 Ontology Annotations

GO Term Gene Name
GO:0000166 IPR004527
GO:0004818 IPR004527
GO:0005524 IPR004527
GO:0006424 IPR004527
GO:0005737 IPR004527

1 Parent Features

DB identifier Type Name
IPR000924 Family Glutamyl/glutaminyl-tRNA synthetase

382 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
172681 D8RME9 PAC:15413541 Selaginella moellendorffii 555  
evm.TU.contig_29182.1 PAC:16429677 Carica papaya 583  
28592.m000288 B9T2L7 PAC:16801181 Ricinus communis 577  
Cucsa.099920.8 PAC:16958495 Cucumis sativus 452  
Cucsa.099920.2 A0A0A0K502 PAC:16958489 Cucumis sativus 573  
Cucsa.099920.1 PAC:16958488 Cucumis sativus 596  
orange1.1g013441m A0A067FQE2 PAC:18099486 Citrus sinensis 443  
orange1.1g008289m A0A067FLS8 PAC:18099485 Citrus sinensis 571  
AT5G64050.1 Q9FEA2 PAC:19670485 Arabidopsis thaliana 570  
Thhalv10003868m V4MJG7 PAC:20198193 Eutrema salsugineum 597  
Thhalv10003914m V4K0V8 PAC:20198194 Eutrema salsugineum 570  
Thhalv10003916m V4K0V8 PAC:20198195 Eutrema salsugineum 570  
Ciclev10028098m V4S5M3 PAC:20814718 Citrus clementina 571  
Lus10022659 PAC:23178554 Linum usitatissimum 597  
Lus10012512 PAC:23143904 Linum usitatissimum 597  
Potri.009G169700.2 PAC:26986263 Populus trichocarpa 453  
Potri.009G169700.3 PAC:26986264 Populus trichocarpa 421  
Potri.009G169700.1 A0A2K1Z963 PAC:26986262 Populus trichocarpa 574  
Gorai.002G014600.4 A0A0D2QYQ4 PAC:26793821 Gossypium raimondii 509  
Gorai.002G014600.5 A0A0D2Q6F4 PAC:26793822 Gossypium raimondii 399  
Gorai.002G014600.3 A0A0D2PZ05 PAC:26793819 Gossypium raimondii 565  
Gorai.002G014600.6 A0A0D2M5D3 PAC:26793823 Gossypium raimondii 360  
Gorai.002G014600.1 A0A0D2NGM0 PAC:26793818 Gossypium raimondii 584  
Gorai.002G014600.2 A0A0D2Q6E7 PAC:26793820 Gossypium raimondii 560  
12396 I0Z7L4 PAC:27393078 Coccomyxa subellipsoidea C-169 514  
210782 PAC:27339335 Micromonas pusilla CCMP1545 1009  
93354 C1DYX8 PAC:27396067 Micromonas sp RCC299 530  
49888 A4RZ66 PAC:27418407 Ostreococcus lucimarinus 546  
Thecc1EG007599t1 A0A061E2R9 PAC:27460276 Theobroma cacao 621  
Migut.B00003.1.p A0A022RCT3 PAC:28947552 Mimulus guttatus 564  

7 Publications

First Author Title Year Journal Volume Pages PubMed ID
            8364025
            8274143
            1852601
            2053131
            9426192
            10673435
            2203971