| Type: | Domain | Name: | DNA binding HTH domain, Fis-type |
| Description: | The Factor for Inversion Stimulation (FIS) protein is a regulator of bacterial functions, and binds specifically to weakly related DNA sequences [,]. It activates ribosomal RNA transcription, and is involved in upstreamactivation of rRNA promoters. The protein has been shown to play a role in the regulation of virulence factorsin both Salmonella typhimuriumand Escherichia coli[]. Some of itsfunctions include inhibition of the initiation of DNA replication from the OriC site, and promotion of Hin-mediated DNA inversion.In its C-terminal extremity, FIS encodes a helix-turn-helix (HTH) DNA- binding motif, which shares a high degree of similarity with other HTHmotifs of more primitive bacterial transcriptional regulators, such as the nitrogen assimilation regulatory proteins (NtrC) from species like Azobacter,Rhodobacter and Rhizobium. This has led to speculation that both evolved from a single common ancestor [].The 3-dimensional structure of the E. coli FIS DNA-binding protein has been determined by means of X-ray diffraction to 2.0A resolution [,]. FIS iscomposed of four alpha-helices tightly intertwined to form a globular dimer with two protruding HTH motifs. The 24 N-terminal amino acids are poorly defined, indicating that they might act as `feelers' suitable for DNA or protein (invertase) recognition []. Other proteins belonging to this subfamily include:E. coli: atoC, hydG, ntrC, fhlA, tyrR,Rhizobium spp.: ntrC, nifA, dctD | Short Name: | HTH_Fis |
| DB identifier | Type | Name |
|---|---|---|
| IPR010113 | Family | Nif-specific regulatory protein |
| IPR014317 | Family | Transcription activator PspF |
| IPR010114 | Family | Signal transduction response regulator, nitrogen regulation NR(I) |
| IPR011785 | Family | Transcription regulator PpsR |
| IPR005412 | Family | DNA-binding protein Fis |
| IPR014264 | Family | Signal transduction response regulator, PEP-CTERM system, putative |
| IPR012704 | Family | Signal transduction response regulator, propionate catabolism, transcriptional regulator PrpR |
