Protein Domain : IPR006463

Type:  Family Name:  tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase MiaB
Description:  This entry represents the the MiaB enzyme and homologues that are responsible for the modification of the isopentenylated adenine-37 base of most bacterial and eukaryotic tRNAs that read codons beginning with uracil (all except tRNA(I,V) Ser). Adenine-37 is next to the anticodon on the 3' side in these tRNA's, and lack of modification at this site leads to an increased spontaneous mutation frequency. Isopentenylated A-37 is modified by methylthiolation at position 2, either by MiaB alone or in concert with a separate methylase yet to be discovered []. MiaB contains a 4Fe-4S cluster which is labile under oxidizing conditions []. Additionally, the sequence is homologous to the biotin synthetase, BioB, which utilises both an iron-sulfur cluster and S-adenosym methionine (SAM) to generate a radical which is responsible for initiating the insertion of sulfur into the substrate []. It is reasonable to surmise that the methyl group of SAM becomes the methyl group of the product, but this has not been shown, and the possibility of a separate methylase exists. These enzymes contain a TRAM domain [] which is believed to be responsible for binding to tRNAs. The enzyme from Thermotoga maritima has been cloned, expressed, spectroscopically characterised and shown to complement the E. coli MiaB enzyme []. Short Name:  MiaB_methiolase
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0 Child Features

4 Contains

DB identifier Type Name
IPR013785 Domain Aldolase-type TIM barrel
IPR006638 Domain Elongator protein 3/MiaB/NifB
IPR013848 Domain Methylthiotransferase, N-terminal
IPR020612 Conserved_site Methylthiotransferase, conserved site

1 Cross References

Identifier
MF_01864

0 Found In

4 GO Annotations

GO Term Gene Name
GO:0016740 IPR006463
GO:0051539 IPR006463
GO:0006400 IPR006463
GO:0005737 IPR006463

4 Ontology Annotations

GO Term Gene Name
GO:0016740 IPR006463
GO:0051539 IPR006463
GO:0006400 IPR006463
GO:0005737 IPR006463

1 Parent Features

DB identifier Type Name
IPR005839 Family Methylthiotransferase

15 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
Araha.1388s0003.1.p PAC:28838843 Arabidopsis halleri 638  
Araha.11756s0141.1.p PAC:28856495 Arabidopsis halleri 427  
Bostr.7867s1498.1.p PAC:30658182 Boechera stricta 635  
Cre11.g467633.t1.1 A0A2K3D7I6 PAC:30775866 Chlamydomonas reinhardtii 569  
SapurV1A.0441s0280.1.p PAC:31432949 Salix purpurea 621  
Spipo24G0021400 PAC:31517580 Spirodela polyrhiza 610  
evm_27.model.AmTr_v1.0_scaffold00002.279 W1NZW5 PAC:31562331 Amborella trichopoda 595  
Pahal.H00560.1 A0A2S3IF01 PAC:32522603 Panicum hallii 598  
Kalax.0809s0010.1.p PAC:32568350 Kalanchoe laxiflora 623  
Kalax.0963s0009.2.p PAC:32587866 Kalanchoe laxiflora 496  
Kalax.0963s0009.1.p PAC:32587865 Kalanchoe laxiflora 623  
Sevir.8G180200.1.p A0A4U6TKB7 PAC:32633176 Setaria viridis 597  
Seita.8G170600.1.p K3ZHT7 PAC:32715025 Setaria italica 597  
Bradi4g14840.2.p A0A2K2CMX1 PAC:32786855 Brachypodium distachyon 760  
Brast10G140800.1.p PAC:32848412 Brachypodium stacei 596  

4 Publications

First Author Title Year Journal Volume Pages PubMed ID
            11313137
            10572129
            11882645
            12766153