Protein Domain : IPR012162

Type:  Family Name:  Polyribonucleotide nucleotidyltransferase
Description:  The eukaryotic exosome and the prokaryotic degradosome are important protein complexes involved in RNA processing and maintaining appropriate RNA levels within the cell []. Both of these complexes contain exoribonucleases (exoRNases) which degrade RNA from the 3' end. The hydrolytic exoRNases produce nucleoside monophosphates, while the phosphorolytic exoRNases add orthophosphate at the cleaved bond to produce nucleoside monophosphates.This entry represents polyribonucleotide nucleotidyltransferase (), also known as polynucleotide phosphorylase (PNPase), found in bacterial and eukaryotic organelle degradosomes. This enzyme can process single-stranded RNA, but is stalled by double-stranded structures such as stem-loops. Structural studies show that PNPase is a trimeric multidomain protein with a central channel []. Each subunit contains duplicated RNase PH-like domains which, though structurally homologous, are thought to be functionally distinct. The first domain is more divergent in sequence than than the second domain and is thought to be involved in the flexible binding of RNA substrate and the formation of the trimer channel structure. The second domain is thought to contain the catalytic site and show exoRNase activity. The catalytic mechanism of the enzyme is not yet known but it seems likely that single-stranded RNA would be threaded through the channel to be processed by the three active sites within the trimer, which would thus be restricted to a single substrate molecule per trimer. PNPase activity would thus be tightly regulated by secondary structural elements within the RNA []. Short Name:  PNPase
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1 Child Features

DB identifier Type Name
IPR014069 Family Guanosine pentaphosphate synthetase I/polyribonucleotide nucleotidyltransferase

8 Contains

DB identifier Type Name
IPR012340 Domain Nucleic acid-binding, OB-fold
IPR020568 Domain Ribosomal protein S5 domain 2-type fold
IPR004088 Domain K Homology domain, type 1
IPR004087 Domain K Homology domain
IPR003029 Domain S1 domain
IPR015847 Domain Exoribonuclease, phosphorolytic domain 2
IPR015848 Domain Polyribonucleotide nucleotidyltransferase, RNA-binding domain
IPR001247 Domain Exoribonuclease, phosphorolytic domain 1

4 Cross Referencess

Identifier
PTHR11252
PIRSF005499
TIGR03591
MF_01595

0 Found In

3 GO Annotations

GO Term Gene Name
GO:0003723 IPR012162
GO:0004654 IPR012162
GO:0006402 IPR012162

3 Ontology Annotations

GO Term Gene Name
GO:0003723 IPR012162
GO:0004654 IPR012162
GO:0006402 IPR012162

0 Parent Features

3256 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
84724 D8R2V3 PAC:15419311 Selaginella moellendorffii 705  
77425 D8QTG9 PAC:15418598 Selaginella moellendorffii 808  
evm.TU.contig_42620.1 PAC:16431772 Carica papaya 41  
evm.model.supercontig_108.35 PAC:16405450 Carica papaya 872  
evm.model.supercontig_161.36 PAC:16410206 Carica papaya 162  
evm.model.supercontig_1931.1 PAC:16412232 Carica papaya 183  
29933.m001410 B9SFA0 PAC:16814138 Ricinus communis 958  
30072.m000941 B9SI65 PAC:16816731 Ricinus communis 948  
Cucsa.338110.1 PAC:16978706 Cucumis sativus 922  
Cucsa.338110.2 PAC:16978707 Cucumis sativus 786  
Cucsa.339890.2 PAC:16978961 Cucumis sativus 866  
Cucsa.339890.3 PAC:16978962 Cucumis sativus 813  
Cucsa.339890.1 A0A0A0LFJ2 PAC:16978960 Cucumis sativus 1032  
Cucsa.339890.4 PAC:16978963 Cucumis sativus 367  
orange1.1g002487m A0A067FS71 PAC:18131835 Citrus sinensis 916  
orange1.1g002054m A0A067EWB2 PAC:18121164 Citrus sinensis 974  
AT5G14580.1 Q9S7G6 PAC:19670993 Arabidopsis thaliana 991  
AT3G03710.1 Q8GZQ3 PAC:19664459 Arabidopsis thaliana 922  
Thhalv10020014m V4M470 PAC:20181727 Eutrema salsugineum 914  
Thhalv10022162m V4NSQ5 PAC:20183318 Eutrema salsugineum 182  
Thhalv10012572m V4LNJ5 PAC:20206188 Eutrema salsugineum 984  
Ciclev10024655m V4T8Z4 PAC:20812095 Citrus clementina 278  
Ciclev10019242m V4U3L7 PAC:20810108 Citrus clementina 645  
Ciclev10024648m V4TTH9 PAC:20812096 Citrus clementina 141  
Ciclev10014159m V4U1N7 PAC:20815384 Citrus clementina 973  
Lus10022250 PAC:23139362 Linum usitatissimum 960  
Lus10001331 PAC:23146554 Linum usitatissimum 201  
Lus10001332 PAC:23146557 Linum usitatissimum 703  
Lus10013636 PAC:23171586 Linum usitatissimum 994  
Lus10008763 PAC:23177852 Linum usitatissimum 921  

3 Publications

First Author Title Year Journal Volume Pages PubMed ID
            17084501
            11080643
            11796219