Protein Domain : IPR002301

Type:  Family Name:  Isoleucine-tRNA ligase
Description:  Isoleucine-tRNA ligase (also known as Isoleucyl-tRNA synthetase)() is an alpha monomer that belongs to class Ia. The enzyme, isoleucine-tRNA ligase, activates not only the cognate substrate L-isoleucine but also the minimally distinct L-valine in the first, aminoacylation step. Then, in a second, "editing" step, the ligase itself rapidly hydrolyses only the valylated products [, ] as shown from the crystal structures. The aminoacyl-tRNA synthetase (also known as aminoacyl-tRNA ligase) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction. These proteins differ widely in size and oligomeric state, and have limited sequence homology []. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric []. Class II aminoacyl-tRNA synthetases share an anti-parallel beta-sheet fold flanked by alpha-helices [], and are mostly dimeric or multimeric, containing at least three conserved regions [, , ]. However, tRNA binding involves an alpha-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan and valine belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, lysine, phenylalanine, proline, serine, and threonine belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c. Short Name:  Ile-tRNA-ligase
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2 Child Features

DB identifier Type Name
IPR023585 Family Isoleucine-tRNA ligase, type 1
IPR023586 Family Isoleucine-tRNA ligase, type 2

4 Contains

DB identifier Type Name
IPR014729 Domain Rossmann-like alpha/beta/alpha sandwich fold
IPR013155 Domain Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding
IPR009008 Domain Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain
IPR001412 Conserved_site Aminoacyl-tRNA synthetase, class I, conserved site

2 Cross Referencess

Identifier
PR00984
TIGR00392

0 Found In

5 GO Annotations

GO Term Gene Name
GO:0000166 IPR002301
GO:0004822 IPR002301
GO:0005524 IPR002301
GO:0006428 IPR002301
GO:0005737 IPR002301

5 Ontology Annotations

GO Term Gene Name
GO:0000166 IPR002301
GO:0004822 IPR002301
GO:0005524 IPR002301
GO:0006428 IPR002301
GO:0005737 IPR002301

0 Parent Features

3256 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
153639 D8S9D7 PAC:15401914 Selaginella moellendorffii 1155  
445517 D8SJ90 PAC:15420275 Selaginella moellendorffii 1155  
437676 D8QNT8 PAC:15419827 Selaginella moellendorffii 1707  
evm.model.supercontig_32.3 PAC:16417407 Carica papaya 531  
29726.m004043 B9RT52 PAC:16806754 Ricinus communis 1102  
29657.m000469 B9SUT5 PAC:16804968 Ricinus communis 1175  
Cucsa.094940.2 A0A0A0KTX5 PAC:16957806 Cucumis sativus 1184  
Cucsa.094940.1 A0A0A0KTX5 PAC:16957805 Cucumis sativus 1184  
Cucsa.253080.2 PAC:16972029 Cucumis sativus 822  
Cucsa.253080.1 PAC:16972028 Cucumis sativus 1097  
orange1.1g000999m A0A067DE71 PAC:18109903 Citrus sinensis 1193  
orange1.1g002784m PAC:18109465 Citrus sinensis 881  
orange1.1g003799m PAC:18109466 Citrus sinensis 794  
AT4G10320.1 F4JLM5 PAC:19644217 Arabidopsis thaliana 1190  
AT5G49030.1 Q8RXK8 PAC:19672750 Arabidopsis thaliana 1093  
AT5G49030.2 Q8RXK8 PAC:19672751 Arabidopsis thaliana 955  
AT5G49030.3 F4K4Q2 PAC:19672749 Arabidopsis thaliana 1279  
Thhalv10027625m V4LW71 PAC:20189717 Eutrema salsugineum 1180  
Thhalv10027624m V4M3E2 PAC:20189716 Eutrema salsugineum 1181  
Thhalv10003559m V4MLQ3 PAC:20199315 Eutrema salsugineum 1090  
Thhalv10003524m V4K4B4 PAC:20199314 Eutrema salsugineum 1276  
Ciclev10004211m V4RG12 PAC:20790583 Citrus clementina 1096  
Lus10043413 PAC:23168015 Linum usitatissimum 997  
Lus10034171 PAC:23154721 Linum usitatissimum 969  
Potri.010G014500.2 PAC:26980333 Populus trichocarpa 981  
Potri.010G014500.3 PAC:26980334 Populus trichocarpa 930  
Potri.010G014500.1 A0A2K1YMA5 PAC:26980332 Populus trichocarpa 1095  
Potri.017G033200.4 PAC:26984654 Populus trichocarpa 977  
Potri.017G033200.7 PAC:26984653 Populus trichocarpa 977  
Potri.017G033200.5 PAC:26984656 Populus trichocarpa 881  

8 Publications

First Author Title Year Journal Volume Pages PubMed ID
            8364025
            8274143
            1852601
            2053131
            10673435
            2203971
            9554847
            10446055