Protein Domain : IPR012695

Type:  Family Name:  2-methylisocitrate lyase
Description:  Propionate is the second most abundant low molecular mass carbon source found in soil, being generated by the degradation of several amino acids, fementation of crabohydrates and the oxidation of odd-chain fatty acids. Many different organisms are capable of metabolising propionate and several distinct pathways for utilising this compound exist, all of which begin with its activation to propionyl-CoA []. Many bacteria metabolise propionate to pyruvate by the 2-methylcitric acid cycle. In the first step, catalysed by methycitrate synthase, oxaloacetate and propionyl-CoA are condensed to form 2-methylcitrate and CoA. The 2-methylcitrate is subsequently converted to 2-methylisocitrate by the combined actions of a dehydratase and an aconitase. In the final step, 2-methylisocitrate lyase cleaves a C-C bond within this compound to form succinate and pyruvate. The succinate is further oxidised to oxaloacetate which re-enters the cycle, while the pyruvate can be used further for either energy generation or biomass synthesis.Proteins in this entry are mostly 2-methylisocitrate lyases. Some of them have been annotated (incorrectly it seems) as the related protein carboxyphosphoenolpyruvate phosphomutase, which is involved in synthesizing the antibiotic bialaphos in Streptomyces hygroscopicus. Structural studies indicate that these enzymes belong to the isocitrate lyase family, binding a Mg(2+) cofactor and forming a TIM barrel fold [, ]. As with other enzymes in this family, substrate-binding induces a conformational change that prevents solvent access to the active site and which is important for catalysis. Short Name:  PrpB
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0 Child Features

2 Contains

DB identifier Type Name
IPR015813 Domain Pyruvate/Phosphoenolpyruvate kinase-like domain
IPR018523 Conserved_site Isocitrate lyase/phosphorylmutase, conserved site

2 Cross Referencess

Identifier
TIGR02317
MF_01939

0 Found In

2 GO Annotations

GO Term Gene Name
GO:0046421 IPR012695
GO:0019629 IPR012695

2 Ontology Annotations

GO Term Gene Name
GO:0046421 IPR012695
GO:0019629 IPR012695

0 Parent Features

5 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
Brdisv1pangenome1007586m.p PAC:33607259 Brachypodium distachyon Pangenome 324  
Brdisv1pangenome1011166m.p PAC:33623991 Brachypodium distachyon Pangenome 497  
Brdisv1BdTR11A1042579m.p PAC:35688752 Brachypodium distachyon BdTR11a 324  
Brdisv1BdTR11A1041103m.p PAC:35689426 Brachypodium distachyon BdTR11a 497  
Brdisv1BdTR11A1041787m.p PAC:35693020 Brachypodium distachyon BdTR11a 260  

3 Publications

First Author Title Year Journal Volume Pages PubMed ID
            14575713
            12706720
            9325432