| Type: | Domain | Name: | HTH CenpB-type DNA-binding domain |
| Description: | The CENPB-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 70-75 amino acids, present in eukaryotic centromere proteins andtransposases. The domain is named after the mammalian major centromere autoantigen B or centromere protein B (CENP-B), which is a fundamentalcentromere component of chromosomes. The N terminus of CENP-B contains two DNA-binding HTH domains, which bind to adjacent major grooves of DNA. TheN terminus of CENP-B is formed by a psq-type HTH domain and C-terminal to this domain lies the CENPB-type HTH domain. These two HTHdomains together bind specifically to a 17-base-pair sequence, the CENP-B box, which occurs in alpha-satellite DNA in human centromeres [].The structure of the CENPB-type HTH domain is composed of three alpha-helices. The second and third helices connected via a turn comprise the helix-turn-helix motif. Helix 3 is termed the recognition helix as itbinds the DNA major groove, like in other HTHs. In CENP-B this domain recognises site 3 of the CENP-B box, while the preceding psq-type HTH binds site 1 of the CENP-B box, and a connecting linker loopbinds in the minor groove of DNA and recognises site 2 [].Some proteins known to contain a CENPB-type HTH domain:Mammalian centromere protein B (CENP-B), associated with the centromere and specifically binding DNA to the CENP-B box.Mammalian jerky protein, involved in epileptic seizures in mice [].Mammalian Pogo transposases [] and tigger transposable elements [].Fission yeast ARS-binding protein 1 (abp1) [] and CENP-B homologue proteins (CBHP-1 and 2), which are centromere proteins [, ].Candida albicans protein PDC2 (Pyruvate DeCarboxylase 2) [, ].Fungal transposases. | Short Name: | HTH_CenpB_DNA-bd_dom |
