| Type: | Domain | Name: | Polymerase, nucleotidyl transferase domain |
| Description: | A small region that overlaps with a nuclear localization signal and binds to the RNA primer contains three aspartates that are essential for catalysis. Sequence and secondary structure comparisons of regions surrounding these aspartates with sequences of other polymerases revealed a significant homology to the palm structure of DNA polymerase beta, terminal deoxynucleotidyltransferase and DNA polymerase IV of Saccharomyces cerevisiae, all members of the family X of polymerases. This homology extends as far as cca: tRNA nucleotidyltransferase and streptomycin adenylyltransferase, an antibiotic resistance factor [, ].Proteins containing this domain include kanamycin nucleotidyltransferase (KNTase) which is a plasmid-coded enzyme responsible for some types of bacterial resistance to aminoglycosides. KNTase inactivates antibiotics by catalysing the addition of a nucleotidyl group onto the drug. In experiments, Mn2+ strongly stimulated this reaction due to a 50-fold lower Ki for 8-azido-ATP in the presence of Mn2+. Mutations of the highly conserved Asp residues 113, 115, and 167, critical for metal binding in the catalytic domain of bovine poly(A) polymerase, led to a strongreduction of cross-linking efficiency, and Mn2+ no longer stimulated the reaction. Mutations in the region of the "helical turn motif" (a domain binding the triphosphate moiety of the nucleotide) and in the suspected nucleotide-binding helix of bovine poly(A) polymeraseimpaired ATP binding and catalysis. The results indicate that ATP is bound in part by the helical turn motif and in part by a region that may be a structural analogue of the fingers domain found in many polymerases. | Short Name: | Polymerase_NTP_transf_dom |
| DB identifier | Type | Name |
|---|---|---|
| IPR001726 | Family | DNA nucleotidylexotransferase (TdT) / DNA-directed DNA/RNA polymerase mu |
| IPR014492 | Family | Poly(A) polymerase |
| IPR006116 | Domain | 2-5-oligoadenylate synthetase, N-terminal |
| IPR010043 | Family | Bifunctional uridylyltransferase/uridylyl-removing enzyme |
| IPR008229 | Family | tRNA nucleotidyltransferase, archaea |
| IPR009185 | Family | Predicted nucleotidyltransferase |
