3 Ontology Annotations
| GO Term | Gene Name |
|---|---|
| GO:0004222 | IPR004387 |
| GO:0006508 | IPR004387 |
| GO:0016021 | IPR004387 |
| Type: | Family | Name: | Peptidase M50, putative membrane-associated zinc metallopeptidase |
| Description: | Metalloproteases are the most diverse of the four main types of protease, with more than 50 families identified to date. In these enzymes, a divalent cation, usually zinc, activates the water molecule. The metal ion is held in place by amino acid ligands, usually three in number. The known metal ligands are His, Glu, Asp or Lys and at least one other residue is required for catalysis, which may play an electrophillic role. Of the known metalloproteases, around half contain an HEXXH motif, which has been shown in crystallographic studies to form part of the metal-binding site []. The HEXXH motif is relatively common, but can be more stringently defined for metalloproteases as 'abXHEbbHbc', where 'a' is most often valine or threonine and forms part of the S1' subsite in thermolysin and neprilysin, 'b' is an uncharged residue, and 'c' a hydrophobic residue. Proline is never found in this site, possibly because it would break the helical structure adopted by this motif in metalloproteases [].This family contains putative zinc metallopeptidases belonging to MEROPS peptidase family M50 (S2P protease family, clan MM). The N-terminal region of contains a perfectly conserved motif HEXGH, where the Glu is the active site and the His residues coordinate the metal cation. The family of bacterial and plant proteins also includes a region that hits the PDZ domain (), found in a number of proteins targeted to the membrane by binding to a peptide ligand []. The family includes EcfE, which is a homologue of human site-2 protease (S2P), a membrane-bound zinc metalloprotease involved in regulated intramembrane proteolysis. In Escherichia coliEcfE activates the sigma(E) pathway of stress response through a site-2 cleavage of anti-sigma(E), RseA. | Short Name: | Pept_M50_Zn |
| GO Term | Gene Name |
|---|---|
| GO:0004222 | IPR004387 |
| GO:0006508 | IPR004387 |
| GO:0016021 | IPR004387 |
