Protein Domain : IPR002052

Type:  Conserved_site Name:  DNA methylase, N-6 adenine-specific, conserved site
Description:  In prokaryotes, the major role of DNA methylation is to protect host DNA against degradation by restriction enzymes. There are 2 major classes of DNA methyltransferase that differ in the nature of the modifications they effect. The members of one class (C-MTases) methylate a ring carbon and form C5-methylcytosine (see PRINTS signature C5METTRFRASE). Members of the second class (N-MTases) methylate exocyclic nitrogens and form either N4-methylcytosine (N4-MTases) or N6-methyladenine (N6-MTases). Both classes of MTase utilise the cofactor S-adenosyl-L-methionine (SAM) as the methyl donor and are active as monomeric enzymes [].N-6 adenine-specific DNA methylases () (A-Mtase) are enzymes that specifically methylate the amino group at the C-6 position of adenines in DNA. Such enzymes are found in the three existing types of bacterial` restriction-modification systems (in type I system the A-Mtase is the product of the hsdM gene, and in type III it is the product of the mod gene). All of these enzymes recognise a specific sequence in DNA and methylate an adenine in that sequence. It has been shown [, , , ] that A-Mtases contain a conserved motif Asp/Asn-Pro-Pro-Tyr/Phe in their N-terminal section, this conserved region could be involved in substrate binding or in the catalytic activity. The structure of N6-MTase TaqI (M.TaqI) has been resolved to 2.4 A []. The molecule folds into 2 domains, an N-terminal catalytic domain, which contains the catalytic and cofactor binding sites, and comprises a central 9-stranded beta-sheet, surrounded by 5 helices; and a C-terminal DNA recognition domain, which is formed by 4 small beta-sheets and 8 alpha-helices. The N- and C-terminal domains form a cleft that accommodates the DNA substrate. A classification of N-MTases has been proposed, based on conserved motif (CM) arrangements []. Three such classes include the D12, D21 and N12 classes. Short Name:  DNA_methylase_N6_adenine_CS
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0 Child Features

0 Contains

1 Cross References

Identifier
PS00092

16 Found Ins

DB identifier Type Name
IPR019874 Family Protein-(glutamine-N5) methyltransferase, release factor-specific
IPR004556 Family Modification methylase HemK
IPR019369 Family Protein-lysine N-methyltransferase Efm5
IPR004398 Family RNA methyltransferase, RsmD
IPR007848 Domain Methyltransferase small domain
IPR002295 Family N6 adenine-specific DNA methyltransferase, D21 class
IPR001091 Family Restriction/modification DNA-methylase
IPR017244 Family Ribosomal RNA large subunit methyltransferase K/L
IPR017127 Family Ribosomal protein L3-specific, glutamine-N5-methyltransferase
IPR012327 Family D12 class N6 adenine-specific DNA methyltransferase
IPR003356 Domain DNA methylase, adenine-specific
IPR011639 Domain Restriction modification methylase Eco57I
IPR012186 Family Adenine modification methylase, M.StsI-type
IPR012263 Family Adenine modification methylase, M.EcoRV-type
IPR014455 Family DNA methylase, N-6 adenine-specific, MK1259 type
IPR021188 Family N6 adenine-specific DNA methyltransferase, TaqI class

3 GO Annotations

GO Term Gene Name
GO:0003676 IPR002052
GO:0008168 IPR002052
GO:0032259 IPR002052

3 Ontology Annotations

GO Term Gene Name
GO:0003676 IPR002052
GO:0008168 IPR002052
GO:0032259 IPR002052

0 Parent Features

0 Proteins

6 Publications

First Author Title Year Journal Volume Pages PubMed ID
            7971991
            7663118
            3248728
            7607512
            3323532
            2541254