| Type: | Conserved_site | Name: | EGF-like calcium-binding, conserved site |
| Description: | A sequence of about forty amino-acid residues found in epidermal growth factor (EGF) has been shown [, , , , ] to be present in a large number of membrane-bound and extracellular, mostly animal, proteins. Many of these proteins require calcium for their biological function and a calcium-binding site has been found at the N terminus of some EGF-like domains []. Calcium-binding may be crucial for numerous protein-protein interactions.For human coagulation factor IX it has been shown [] that the calcium-ligands form a pentagonal bipyramid. The first, third and fourth conserved negatively charged or polar residues are side chain ligands. The latter is possibly hydroxylated (see aspartic acid and asparagine hydroxylation site) []. A conserved aromatic residue, as well as the second conserved negative residue, are thought to be involved in stabilising the calcium-binding site.As in non-calcium binding EGF-like domains, there are six conserved cysteines and the structure of both types is very similar as calcium-binding induces only strictly local structural changes [].+------------------+ +---------+ | | | |nxnnC-x(3,14)-C-x(3,7)-CxxbxxxxaxC-x(1,6)-C-x(8,13)-Cx | | +------------------+ 'n': negatively charged or polar residue [DEQN]'b': possibly beta-hydroxylated residue [DN] 'a': aromatic amino acid'C': cysteine, involved in disulphide bond 'x': any amino acid. | Short Name: | EGF_Ca-bd_CS |
| DB identifier | Type | Name |
|---|---|---|
| IPR000742 | Domain | EGF-like domain |
| IPR001881 | Domain | EGF-like calcium-binding domain |
| IPR016317 | Family | Pro-epidermal growth factor |
| IPR016316 | Family | Thrombomodulin |
| IPR008297 | Family | Notch |
| IPR003056 | Family | GPCR, family 2, CD97 antigen |
| IPR017048 | Family | Fibulin-1 |
| IPR011398 | Family | Fibrillin |
| IPR015446 | Family | Bone morphogenetic protein 1/tolloid-like protein |
| IPR012224 | Family | Peptidase S1A, coagulation factor VII/IX/X/C/Z |
