Protein Domain : IPR020615

Type:  Active_site Name:  Thiolase, acyl-enzyme intermediate active site
Description:  Two different types of thiolase [, , ] are found both in eukaryotes and in prokaryotes: acetoacetyl-CoA thiolase () and 3-ketoacyl-CoA thiolase (). 3-ketoacyl-CoA thiolase (also called thiolase I) has a broad chain-length specificity for its substrates and is involved in degradative pathways such as fatty acid beta-oxidation. Acetoacetyl-CoA thiolase (also called thiolase II) is specific for the thiolysis of acetoacetyl-CoA and involved in biosynthetic pathways such as poly beta-hydroxybutyrate synthesis or steroid biogenesis.In eukaryotes, there are two forms of 3-ketoacyl-CoA thiolase: one located in the mitochondrion and the other in peroxisomes.Mammalian nonspecific lipid-transfer protein (nsL-TP) (also known as sterol carrier protein 2) is a protein which seems to exist in two different forms: a 14 Kd protein (SCP-2) and a larger 58 Kd protein (SCP-x). The former is found in the cytoplasm or the mitochondria and is involved in lipid transport; the latter is found in peroxisomes. The C-terminal part of SCP-x is identical to SCP-2 while the N-terminal portion is evolutionary related to thiolases [].There are two conserved cysteine residues important for thiolase activity. The signature pattern for this entry contains the first conserved cysteine located in the N-terminal section of the enzymes, which is involved in the formation of an acyl-enzyme intermediate; the second located at the C-terminal extremity is the active site base involved in deprotonation in the condensation reaction []. Short Name:  Thiolase_acyl_enz_int_AS

0 Child Features

0 Contains

1 Cross References

Identifier
PS00098

6 Found Ins

DB identifier Type Name
IPR016039 Domain Thiolase-like
IPR020616 Domain Thiolase, N-terminal
IPR002155 Family Thiolase
IPR012793 Family Beta-ketoadipyl CoA thiolase
IPR012806 Family Acetyl-CoA C-acyltransferase FadI
IPR012805 Family Acetyl-CoA C-acyltransferase FadA

2 GO Annotations

GO Term Gene Name
GO:0016747 IPR020615
GO:0008152 IPR020615

2 Ontology Annotations

GO Term Gene Name
GO:0016747 IPR020615
GO:0008152 IPR020615

0 Parent Features

0 Proteins

4 Publications

First Author Title Year Journal Volume Pages PubMed ID
            1755959
            2191949
            1354266
            15589695