3 Ontology Annotations
| GO Term | Gene Name |
|---|---|
| GO:0015503 | IPR020884 |
| GO:0006813 | IPR020884 |
| GO:0005886 | IPR020884 |
| Type: | Family | Name: | Glutathione-regulated potassium-efflux system protein KefB |
| Description: | The CPA2 family is a moderately large family (over 100 sequenced members) from bacteria, archaea and eukaryotes. Among the functionally well-characterised members of the family are (1) the KefB/KefC K+ efflux proteins of Escherichia coliwhich may be capable of catalysing both K+/H+ antiport and K+ uniport, depending on conditions [, ], (2) the Na+/H+ antiporter of Enterococcus hirae[] and (3) the K+/H+ antiporter of Saccharomyces cerevisiae. It has been proposed that under normal physiological conditions, these proteins may function by essentially the same mechanism [].KefC and KefB of E. coli are responsible for glutathione-gated K+ efflux [, ]. Each of these proteins consists of a transmembrane hydrophobic N-terminal domain, and a less well-conserved C-terminal hydrophilic domain. Each protein interacts with a second protein encoded by genes that overlap the gene encoding the primary transporter. The KefB ancillary protein is YheR. The ancillary proteins stimulate transport activity about 10-fold []. They are important for cell survival during exposure to toxic metabolites, possibly because they can release K+, allowing H+ uptake. Activation of the KefB or KefC K+ efflux system only occurs in the presence of glutathione and a reactive electrophile such as methylglyoxal or N-ethylmaleimide. Formation of the methylglyoxal-glutathione conjugate, S-lactoylglutathione, is catalysed by glyoxalase I, and S-lactoylglutathione activates KefB and KefC []. H+ uptake (acidification of the cytoplasm) accompanying or following K+ efflux may serve as a further protective mechanism against electrophile toxicity [, ]. Inhibition of transport by glutathione is enhanced by NADH []. This entry represents the Glutathione-regulated potassium-efflux system protein KefB. | Short Name: | K_H_efflux_KefB |
| GO Term | Gene Name |
|---|---|
| GO:0015503 | IPR020884 |
| GO:0006813 | IPR020884 |
| GO:0005886 | IPR020884 |
