Protein Domain : IPR000485

Type:  Domain Name:  AsnC-type HTH domain
Description:  The asnC-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 60 amino acids present in transcription regulators of the asnC/lrp family. This family of prokaryotic regulators is named after Escherichia coli asnC and Leucine-responsive Regulatory Protein (lrp), which are a regulator of asparagine synthesis and a global regulator of various operons, respectively []. AsnC/lrp-like proteins are present in bacteria and archaea []. The DNA-binding asnC-type HTH domain occurs usually in the N-terminal part. The C-terminal part can contain an effector-binding domain and/or an oligomerisation domain. The crystal structure of hyperthermophilic archaeal lrpA shows that the N-terminal, DNA binding domain contains a core of three alpha-helices, followed by a single beta-strand, which connects as a flexible hinge to the effector binding domain. The second and third helices, connected via a turn, comprise the helix-turn-helix motif. Helix 3 is termed the recognition helix as it binds the DNA major groove, like in other HTHs. Most E. coli lrp DNA binding mutants are positioned in the lrpA structure on the HTH and three are on the hinge [].Proteins known to contain an asnC-type HTH domain include: Escherichia coli Leucine-responsive Regulatory Protein (lrp), a global transcriptional regulator of 35-75 different genes involved in amino acid biosynthesis, amino acid degradation, transport or pili formation. Binding of leucine by lrp can stimulate or reduce the regulatory effect of activation for some operons or repression for others. Lrp negatively autoregulates the lrp gene, independently of leucine.Salmonella typhimurium lrp, a global leucine-responsive regulator involved in branched-chain amino acid biosynthesis, pili formation and plasmid virulence.Escherichia coli asnC, a specific asparagine-dependent transcriptional activator of asparagine biosynthesis. AsnC is also an asparagine-independent repressor of its own transcription.Pseudomonas putida bkdR, a specific autoregulatory transcriptional regulator, involved in catabolism of branched-chain amino acids.Agrobacterium tumefaciens putR, a specific proline-responsive regulator of proline catabolism.Bacillus subtilis lrpA/lrpB and lrpC, transcriptional regulators involved in serine-glycine interconversion, sporulation and amino acid metabolism. LrpC binds to a specific DNA structure and wraps and overwinds the DNA [].Bacillus subtilis azlB, a specific transcriptional repressor of branched-chain amino acid transport.Pyrococcus furiosus lrpA, a putative lrp with negative autoregulation.Zymomonas mobilis grp, a repressor of the glutamate uptake operon. Short Name:  AsnC-type_HTH_dom
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0 Child Features

1 Contains

DB identifier Type Name
IPR019885 Conserved_site Transcription regulator HTH, AsnC-type, conserved site

3 Cross Referencess

Identifier
PF13404
PR00033
PS50956

1 Found In

DB identifier Type Name
IPR019888 Family Transcription regulator AsnC-type

2 GO Annotations

GO Term Gene Name
GO:0043565 IPR000485
GO:0006355 IPR000485

2 Ontology Annotations

GO Term Gene Name
GO:0043565 IPR000485
GO:0006355 IPR000485

1 Parent Features

DB identifier Type Name
IPR011991 Domain Winged helix-turn-helix DNA-binding domain

5 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
30316.m000024 B9TJJ9 PAC:16824337 Ricinus communis 172  
33925.m000029 B9TF53 PAC:16826034 Ricinus communis 160  
27712.m000038 B9TBH7 PAC:16798993 Ricinus communis 152  
Pp3s32_30V3.1.p PAC:32948416 Physcomitrium patens 2661  
Brdisv1pangenome1006963m.p PAC:33619841 Brachypodium distachyon Pangenome 731  

4 Publications

First Author Title Year Journal Volume Pages PubMed ID
            2040596
            7770911
            11230123
            12458218