Protein Domain : IPR009377

Type:  Family Name:  Ethanolamine utilisation EutA
Description:  Proteins in this entry are EutA ethanolamine utilization proteins, reactivating factors for ethanolamine ammonia lyase, encoded by the ethanolamine utilization eut operon.The holoenzyme of adenosylcobalamin-dependent ethanolamine ammonia-lyase (EutBC, , ), which is part of the ethanolamine utilization pathway [, , ], undergoes suicidal inactivation during catalysis as well as inactivation in the absence of substrate. The inactivation involves the irreversible cleavage of the Co-C bond of the coenzyme. The inactivated holoenzyme undergoes rapid and continuous reactivation in the presence of ATP, Mg2+, and free adenosylcobalamin in permeabilised cells (in situ), homogenate, and cell extracts of Escherichia coli. The EutA protein is essential for reactivation. It was demonstrated with purified recombinant EutA that both the suicidally inactivated and O2-inactivated holoethanolamine ammonia lyase underwent rapid reactivation in vitro by EutA in the presence of adenosylcobalamin, ATP, and Mg2+ []. The inactive enzyme-cyanocobalamin complex was also activated in situ and in vitro by EutA under the same conditions. Thus EutA is believed to be the only component of the reactivating factor for ethanolamine ammonia lyase. Reactivation and activation occur through the exchange of modified coenzyme for free intact adenosylcobalamin [].Bacteria that harbor the ethanolamine utilization pathway can use ethanolamine as a source of carbon and nitrogen. For more information on the ethanolamine utilization pathway, please see , . Short Name:  Ethanolamine_utilisation_EutA
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0 Child Features

0 Contains

2 Cross Referencess

Identifier
PF06277
PIRSF012293

0 Found In

0 GO Annotation

0 Ontology Annotations

0 Parent Features

4 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
PGSC0003DMP400042839 PAC:37438013 Solanum tuberosum 458  
PGSC0003DMP400042841 PAC:37438012 Solanum tuberosum 461  
evm.model.Scaffold_636.477 PAC:37855257 Coffea arabica 560  
evm.model.Scaffold_632.304 PAC:37859647 Coffea arabica 560  

4 Publications

First Author Title Year Journal Volume Pages PubMed ID
            10464203
            11160088
            2656649
            15466038