Protein Domain : IPR011826

Type:  Family Name:  Homoaconitase/3-isopropylmalate dehydratase, large subunit, prokaryotic
Description:  3-isopropylmalate dehydratase (or isopropylmalate isomerase; ) catalyses the stereo-specific isomerisation of 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate. This enzyme performs the second step in the biosynthesis of leucine, and is present in most prokaryotes and many fungal species. The prokaryotic enzyme is a heterodimer composed of a large (LeuC) and small (LeuD) subunit, while the fungal form is a monomeric enzyme. Both forms of isopropylmalate are related and are part of the larger aconitase family []. Aconitases are mostly monomeric proteins which share four domains in common and contain a single, labile [4Fe-4S]cluster. Three structural domains (1, 2 and 3) are tightly packed around the iron-sulphur cluster, while a fourth domain (4) forms a deep active-site cleft. The prokaryotic enzyme is encoded by two adjacent genes, leuC and leuD, corresponding to aconitase domains 1-3 and 4 respectively [, ]. LeuC does not bind an iron-sulphur cluster. It is thought that some prokaryotic isopropylamalate dehydrogenases can also function as homoaconitase , converting cis-homoaconitate to homoisocitric acid in lysine biosynthesis []. Homoaconitase has been identified in higher fungi (mitochondria) and several archaea and one thermophilic species of bacteria, Thermus thermophilus[]. This entry represents the large subunit of 3-isopropylmalate dehydratase (LeuC), as well as homoaconitase enzymes, from prokaryotes. Homoaconitase, aconitase and 3-isopropylmalate dehydratase have similar overall structures and domain organisation []. All are dehydratases that bind a [4Fe-4S]-cluster. Short Name:  HAcnase/IPMdehydase_lsu_prok
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1 Child Features

DB identifier Type Name
IPR011823 Family 3-isopropylmalate dehydratase, large subunit, bacteria

4 Contains

DB identifier Type Name
IPR015931 Domain Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3
IPR001030 Domain Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain
IPR015932 Domain Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 2
IPR018136 Binding_site Aconitase family, 4Fe-4S cluster binding site

2 Cross Referencess

Identifier
TIGR02086
MF_01027

0 Found In

3 GO Annotations

GO Term Gene Name
GO:0003861 IPR011826
GO:0051539 IPR011826
GO:0009098 IPR011826

3 Ontology Annotations

GO Term Gene Name
GO:0003861 IPR011826
GO:0051539 IPR011826
GO:0009098 IPR011826

1 Parent Features

DB identifier Type Name
IPR006251 Family Homoaconitase/3-isopropylmalate dehydratase, large subunit

0 Proteins

5 Publications

First Author Title Year Journal Volume Pages PubMed ID
            9020582
            9813279
            15522288
            1400210
            16524361