Protein Domain : IPR002057

Type:  Conserved_site Name:  Isopenicillin N synthase, conserved site
Description:  Isopenicillin N synthase (IPNS) is a nonhaem-Fe2+-dependent enzyme that belongs to a class of nonhaem Fe2+-containing enzymes, which includes 2-oxoglutarate-dependent dioxygenases, 2-oxoglutarate-dependent hydroxy-lases, and enzymes involved in ethylene formation and anthocyaninidin biosynthesis. IPNS catalyses the stereospecific formation of the beta-lactam and thiazolidine rings of IPN via a 4-electron oxidation of the tripeptide ACV. The enzyme uses, as the sole electron acceptor, one molecule ofdioxygen, which is completely reduced to two molecules of water. The oxygen stoichiometry shown by IPNS is the same as that for cytochrome C oxidase,but is unusual for nonhaem-iron-containing enzymes, which typically catalyse the transfer of one or both atoms of dioxygen into their substrates. IPNSthus provides an interesting contrast to dioxygenase enzymes. Two cysteines are conserved in fungal and bacterial IPNS sequences; these may be involved in iron-binding and/orsubstrate-binding.Cephalosporium acremoniumDAOCS/DACS is a bifunctional enzyme involved in cephalosporin biosynthesis. The DAOCS domain, which is structurally related toIPNS, catalyzes the step from penicillin N to deacetoxy-cephalosporin C - used as a substrate by DACS to form deacetylcephalosporin C. Streptomyces clavuligeruspossesses a monofunctional DAOCS enzyme (gene cefE) [] alsorelated to IPNS. Short Name:  Isopenicillin-N_synth_CS
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0 Child Features

0 Contains

2 Cross Referencess

Identifier
PS00185
PS00186

2 Found Ins

DB identifier Type Name
IPR005123 Domain Oxoglutarate/iron-dependent dioxygenase
IPR002283 Family Isopenicillin N synthase

4 GO Annotations

GO Term Gene Name
GO:0005506 IPR002057
GO:0016491 IPR002057
GO:0009058 IPR002057
GO:0055114 IPR002057

4 Ontology Annotations

GO Term Gene Name
GO:0005506 IPR002057
GO:0016491 IPR002057
GO:0009058 IPR002057
GO:0055114 IPR002057

0 Parent Features

0 Proteins

1 Publications

First Author Title Year Journal Volume Pages PubMed ID
            2644235