Protein Domain : IPR007560

Type:  Domain Name:  Restriction endonuclease type IV, Mrr
Description:  There are four classes of restriction endonucleases: types I, II,III and IV. All types of enzymes recognise specific short DNA sequences and carry out the endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates. They differ in their recognition sequence, subunit composition, cleavage position, and cofactor requirements [, ], as summarised below:Type I enzymes () cleave at sites remote from recognition site; require both ATP and S-adenosyl-L-methionine to function; multifunctional protein with both restriction and methylase () activities.Type II enzymes () cleave within or at short specific distances from recognition site; most require magnesium; single function (restriction) enzymes independent of methylase.Type III enzymes () cleave at sites a short distance from recognition site; require ATP (but doesn't hydrolyse it); S-adenosyl-L-methionine stimulates reaction but is not required; exists as part of a complex with a modification methylase methylase ().Type IV enzymes target methylated DNA.This entry represents Mrr, a type IV restriction endonuclease involved in the acceptance of modified foreign DNA, restricting both adenine- and cytosine-methylated DNA. Plasmids carrying HincII, HpaI, and TaqI R and M genes are severely restricted in Escherichia colistrains that are Mrr+ []. Mrr appears to be the final effector of the bacterial SOS response, which is not only a vital reply to DNA damage but also constitutes an essential mechanism for the generation of genetic variability that in turn fuels adaptation and resistance development in bacterial populations []. Mrr possesses a cleavage domain that is similar to that found in type II restriction enzymes, however it has an unusual glutamine residue at the central position of the (D/E)-(D/E)XK hallmark of the active site []. Short Name:  Restrct_endonuc_IV_Mrr
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0 Child Features

0 Contains

1 Cross References

Identifier
PF04471

1 Found In

DB identifier Type Name
IPR016984 Family Uncharacterised conserved protein UCP031853

3 GO Annotations

GO Term Gene Name
GO:0003677 IPR007560
GO:0004519 IPR007560
GO:0009307 IPR007560

3 Ontology Annotations

GO Term Gene Name
GO:0003677 IPR007560
GO:0004519 IPR007560
GO:0009307 IPR007560

1 Parent Features

DB identifier Type Name
IPR011335 Domain Restriction endonuclease type II-like

4 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
35941.m000016 B9TD46 PAC:16826800 Ricinus communis 512  
23738 I0YXI5 PAC:27392551 Coccomyxa subellipsoidea C-169 202  
Cre09.g403219.t1.1 A0A2K3DF68 PAC:30780365 Chlamydomonas reinhardtii 202  
Joasc.12G075600.4.p PAC:45774783 Joinvillea ascendens 131  

5 Publications

First Author Title Year Journal Volume Pages PubMed ID
            15121719
            12665693
            1650347
            11313145
            16313623