Protein Domain : IPR016202

Type:  Family Name:  Deoxyribonuclease I
Description:  Deoxyribonuclease I (DNase I) () [] is a vertebrate enzyme which catalyzes the endonucleolytic cleavage of double-stranded DNA to 5'- phosphodinucleotide and 5'-phosphooligonucleotide end-products. DNase I is an enzyme involved in DNA degradation; it is normally secreted outside of the cell but seems to be able to gain access to the nucleus where it is involved in cell death by apoptosis [].As shown in the following schematic representation, DNase I is a glycoprotein of about 260 residues with two conserved disulphide bonds. +-+ +--------+ | | | |xxxxxxxxxxxxxxxxx#xxxxxxCxCxxxxx#xxxxxxxxxCxxxxxxxxCxxxxxxxxxxxxx 'C': conserved cysteine involved in a disulphide bond.'#': active site residue. DNase I has a pH-optimum around 7.5 and requires calcium and magnesium for full activity. It causes single strand nicks in duplex DNA. A proton acceptor-donor chain composed of an histidine and a glutamic acid produce a nucleophilic hydroxyl ion from water, which cleaves the 3'-P-O bond [].DNase I forms a 1:1 complex with G-actin, resulting in the inhibition of DNase activity and loss of the ability of G-actin to polymerise into fibres [].DNase I has been used in the treatment of lung problems in patients with cystic fibrosis: here it acts by degrading DNA found in purulent lung secretions, reducing their viscosity and making it easier for the patient to breathe [].The sequence of DNase I is evolutionary related to that of human muscle-specific DNase-like protein and human proteins DHP1 and DHP2. However, the first disulphide bond of DNase I is not conserved in these proteins.This entry represents DNaseI and related proteins such as DNase gamma. Short Name:  DNase_I
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0 Child Features

2 Contains

DB identifier Type Name
IPR005135 Domain Endonuclease/exonuclease/phosphatase
IPR018057 Active_site Deoxyribonuclease I, active site

4 Cross Referencess

Identifier
PTHR11371
PIRSF000988
PR00130
SM00476

0 Found In

2 GO Annotations

GO Term Gene Name
GO:0004536 IPR016202
GO:0006308 IPR016202

2 Ontology Annotations

GO Term Gene Name
GO:0004536 IPR016202
GO:0006308 IPR016202

0 Parent Features

3 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
30786.m000015 B9TE21 PAC:16824620 Ricinus communis 510  
55978.m000020 B9TMR1 PAC:16828887 Ricinus communis 111  
27983.m000078 B9T8V7 PAC:16799681 Ricinus communis 962  

5 Publications

First Author Title Year Journal Volume Pages PubMed ID
            8428592
            3713845
            3352748
            2395459
            2251263