Protein Domain : IPR004188

Type:  Domain Name:  Phenylalanine-tRNA ligase, class II, N-terminal
Description:  Phenylalanine-tRNA ligase (also known as phenylalanyl-tRNA synthetase) from Thermus thermophilushas an alpha 2 beta 2 type quaternary structure and is one of the most complicated members of the ligase family. Identification of phenylalanine-tRNA ligase a member of class II aaRSs was based only on sequence alignment of the small alpha-subunit with other ligases []. This is the N-terminal domain of phenylalanine-tRNA ligase.The aminoacyl-tRNA synthetase (also known as aminoacyl-tRNA ligase) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction. These proteins differ widely in size and oligomeric state, and have limited sequence homology []. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric []. Class II aminoacyl-tRNA synthetases share an anti-parallel beta-sheet fold flanked by alpha-helices [], and are mostly dimeric or multimeric, containing at least three conserved regions [, , ]. However, tRNA binding involves an alpha-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan and valine belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, lysine, phenylalanine, proline, serine, and threonine belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c. Short Name:  Phe-tRNA_ligase_II_N
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0 Child Features

0 Contains

1 Cross References

Identifier
PF02912

2 Found Ins

DB identifier Type Name
IPR004529 Family Phenylalanyl-tRNA synthetase, class IIc, alpha subunit
IPR002319 Domain Phenylalanyl-tRNA synthetase

5 GO Annotations

GO Term Gene Name
GO:0000166 IPR004188
GO:0004826 IPR004188
GO:0005524 IPR004188
GO:0006432 IPR004188
GO:0005737 IPR004188

5 Ontology Annotations

GO Term Gene Name
GO:0000166 IPR004188
GO:0004826 IPR004188
GO:0005524 IPR004188
GO:0006432 IPR004188
GO:0005737 IPR004188

1 Parent Features

DB identifier Type Name
IPR010978 Domain tRNA-binding arm

2 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
Araha.11756s0349.1.p PAC:28856362 Arabidopsis halleri 329  
Pum0107s0029.1.p A0A1X6PCC0 PAC:38017035 Porphyra umbilicalis 418  

7 Publications

First Author Title Year Journal Volume Pages PubMed ID
            8364025
            8274143
            1852601
            2053131
            10673435
            2203971
            8199244