Protein Domain : IPR002312

Type:  Family Name:  Aspartyl/Asparaginyl-tRNA synthetase, class IIb
Description:  Aspartyl tRNA synthetase is an alpha2 dimer that belongs to class IIb. Structural analysis combined with mutagenesis and enzymology data on the yeast enzyme point to a tRNA binding process that starts by a recognition event between the tRNA anticodon loop and the synthetase anticodon binding module []. The aminoacyl-tRNA synthetase (also known as aminoacyl-tRNA ligase) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction. These proteins differ widely in size and oligomeric state, and have limited sequence homology []. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric []. Class II aminoacyl-tRNA synthetases share an anti-parallel beta-sheet fold flanked by alpha-helices [], and are mostly dimeric or multimeric, containing at least three conserved regions [, , ]. However, tRNA binding involves an alpha-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan and valine belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, lysine, phenylalanine, proline, serine, and threonine belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c. Short Name:  Asp/Asn-tRNA-synth_IIb

3 Child Features

DB identifier Type Name
IPR004523 Family Aspartyl-tRNA synthetase, archaeal-type
IPR004524 Family Aspartate-tRNA ligase, bacterial/mitochondrial-type
IPR004522 Family Asparagine-tRNA ligase

5 Contains

DB identifier Type Name
IPR012340 Domain Nucleic acid-binding, OB-fold
IPR006195 Domain Aminoacyl-tRNA synthetase, class II
IPR004364 Domain Aminoacyl-tRNA synthetase, class II (D/K/N)
IPR004365 Domain OB-fold nucleic acid binding domain, AA-tRNA synthetase-type
IPR004115 Domain GAD-like domain

1 Cross References


0 Found In

4 GO Annotations

GO Term Gene Name Organism
GO:0000166 IPR002312
GO:0004812 IPR002312
GO:0005524 IPR002312
GO:0006418 IPR002312

1 Parent Features

DB identifier Type Name
IPR018150 Family Aminoacyl-tRNA synthetase, class II (D/K/N)-like

965 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
84215 D8R3T7 PAC:15415533 Selaginella moellendorffii 521  
140340 D8QTI9 PAC:15405936 Selaginella moellendorffii 555  
92995 D8RG18 PAC:15422242 Selaginella moellendorffii 563  
418417 D8S5M5 PAC:15406633 Selaginella moellendorffii 531  
evm.model.supercontig_29.159 PAC:16416187 Carica papaya 375  
evm.model.supercontig_37.195 PAC:16418653 Carica papaya 544  
evm.model.supercontig_78.58 PAC:16426236 Carica papaya 572  
29428.m000317 B9SY11 PAC:16802834 Ricinus communis 607  
29910.m000926 B9SBT1 PAC:16813113 Ricinus communis 540  
29912.m005417 B9RKD1 PAC:16813294 Ricinus communis 364  
29916.m000518 B9SSE8 PAC:16813512 Ricinus communis 187  
30099.m001677 B9S7X4 PAC:16817698 Ricinus communis 668  
30099.m001711 B9S808 PAC:16817732 Ricinus communis 206  
30099.m001712 B9S809 PAC:16817733 Ricinus communis 328  
Cucsa.387780.1 A0A0A0K8G1 PAC:16982763 Cucumis sativus 613  
Cucsa.387780.2 PAC:16982764 Cucumis sativus 508  
Cucsa.089220.1 PAC:16957111 Cucumis sativus 689  
Cucsa.089220.2 PAC:16957112 Cucumis sativus 689  
Cucsa.289040.1 A0A0A0LYN5 PAC:16975160 Cucumis sativus 572  
Cucsa.298550.1 A0A0A0KT15 PAC:16975473 Cucumis sativus 670  
Cucsa.310780.1 PAC:16976582 Cucumis sativus 545  
Cucsa.320930.1 A0A0A0L5I1 PAC:16977417 Cucumis sativus 562  
Cucsa.320930.2 PAC:16977418 Cucumis sativus 525  
Cucsa.320930.3 PAC:16977419 Cucumis sativus 422  
GSVIVT01015254001 PAC:17826044 Vitis vinifera 517  
GSVIVT01037036001 D7T3N6 PAC:17841781 Vitis vinifera 657  
GSVIVT01035583001 PAC:17840715 Vitis vinifera 404  
GSVIVT01004706001 D7U3N1 PAC:17819103 Vitis vinifera 584  
orange1.1g011197m A0A067F5M9 PAC:18094339 Citrus sinensis 491  
orange1.1g011364m A0A067FE00 PAC:18094340 Citrus sinensis 487  

7 Publications

First Author Title Year Journal Volume Pages PubMed ID