Protein Domain : IPR002301

Type:  Family Name:  Isoleucine-tRNA ligase
Description:  Isoleucine-tRNA ligase (also known as Isoleucyl-tRNA synthetase)() is an alpha monomer that belongs to class Ia. The enzyme, isoleucine-tRNA ligase, activates not only the cognate substrate L-isoleucine but also the minimally distinct L-valine in the first, aminoacylation step. Then, in a second, "editing" step, the ligase itself rapidly hydrolyses only the valylated products [, ]as shown from the crystal structures. The aminoacyl-tRNA synthetase (also known as aminoacyl-tRNA ligase)catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction. These proteins differ widely in size and oligomeric state, and have limited sequence homology []. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric []. Class II aminoacyl-tRNA synthetases share an anti-parallel beta-sheet fold flanked by alpha-helices [], and are mostly dimeric or multimeric, containing at least three conserved regions [, , ]. However, tRNA binding involves an alpha-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan and valine belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, lysine, phenylalanine, proline, serine, and threonine belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c. Short Name:  Ile-tRNA-ligase

2 Child Features

DB identifier Type Name
IPR023586 Family Isoleucine-tRNA ligase, type 2
IPR023585 Family Isoleucine-tRNA ligase, type 1

4 Contains

DB identifier Type Name
IPR014729 Domain Rossmann-like alpha/beta/alpha sandwich fold
IPR013155 Domain Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding
IPR009008 Domain Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain
IPR001412 Conserved_site Aminoacyl-tRNA synthetase, class I, conserved site

2 Cross Referencess


0 Found In

5 GO Annotations

GO Term Gene Name Organism
GO:0000166 IPR002301
GO:0004822 IPR002301
GO:0005524 IPR002301
GO:0006428 IPR002301
GO:0005737 IPR002301

0 Parent Features

376 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
445517 D8SJ90 PAC:15420275 Selaginella moellendorffii 1155  
153639 D8S9D7 PAC:15401914 Selaginella moellendorffii 1155  
437676 D8QNT8 PAC:15419827 Selaginella moellendorffii 1707  
evm.model.supercontig_32.3 PAC:16417407 Carica papaya 531  
29657.m000469 B9SUT5 PAC:16804968 Ricinus communis 1175  
29726.m004043 B9RT52 PAC:16806754 Ricinus communis 1102  
Cucsa.094940.1 A0A0A0KTX5 PAC:16957805 Cucumis sativus 1184  
Cucsa.094940.2 A0A0A0KTX5 PAC:16957806 Cucumis sativus 1184  
Cucsa.253080.1 PAC:16972028 Cucumis sativus 1097  
Cucsa.253080.2 PAC:16972029 Cucumis sativus 822  
GSVIVT01010735001 PAC:17822807 Vitis vinifera 1139  
GSVIVT01017762001 PAC:17827882 Vitis vinifera 1221  
orange1.1g002784m PAC:18109465 Citrus sinensis 881  
orange1.1g003799m PAC:18109466 Citrus sinensis 794  
orange1.1g000999m A0A067DE71 PAC:18109903 Citrus sinensis 1193  
AT5G49030.3 F4K4Q2 PAC:19672749 Arabidopsis thaliana 1279  
AT4G10320.1 F4JLM5 PAC:19644217 Arabidopsis thaliana 1190  
AT5G49030.2 Q8RXK8 PAC:19672751 Arabidopsis thaliana 955  
AT5G49030.1 Q8RXK8 PAC:19672750 Arabidopsis thaliana 1093  
Thhalv10027624m V4M3E2 PAC:20189716 Eutrema salsugineum 1181  
Thhalv10027625m V4LW71 PAC:20189717 Eutrema salsugineum 1180  
Thhalv10003524m V4K4B4 PAC:20199314 Eutrema salsugineum 1276  
Thhalv10003559m V4MLQ3 PAC:20199315 Eutrema salsugineum 1090  
Ciclev10018576m V4U2F7 PAC:20806874 Citrus clementina 1161  
Ciclev10004211m V4RG12 PAC:20790583 Citrus clementina 1096  
Carubv10015963m R0I3W8 PAC:20899762 Capsella rubella 1180  
Carubv10000080m R0H8A6 PAC:20908043 Capsella rubella 1144  
Carubv10025780m R0GKJ2 PAC:20912473 Capsella rubella 1090  
MDP0000176251 PAC:22621881 Malus domestica 1105  
MDP0000149868 PAC:22660334 Malus domestica 1089  

8 Publications

First Author Title Year Journal Volume Pages PubMed ID