Protein Domain : IPR004500

Type:  Family Name:  Prolyl-tRNA synthetase, class IIa, bacterial-type
Description:  The aminoacyl-tRNA synthetase (also known as aminoacyl-tRNA ligase) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction. These proteins differ widely in size and oligomeric state, and have limited sequence homology []. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric []. Class II aminoacyl-tRNA synthetases share an anti-parallel beta-sheet fold flanked by alpha-helices [], and are mostly dimeric or multimeric, containing at least three conserved regions [, , ]. However, tRNA binding involves an alpha-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan and valine belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, lysine, phenylalanine, proline, serine, and threonine belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c.Prolyl-tRNA synthetase belongs to class IIa. Prolyl-tRNA synthetase () exists in two forms, which are loosely related. The first form is present in the majority of eubacteria species. The second one, present in some eubacteria, is essentially present in archaea and eukaryota. This entry represents the first form of prolyl-tRNA synthetase. Short Name:  Pro-tRNA-synth_IIa_bac-type

2 Child Features

DB identifier Type Name
IPR023716 Family Proline-tRNA ligase, class IIa, type 2
IPR023717 Family Prolyl-tRNA synthetase, class IIa, type 1

4 Contains

DB identifier Type Name
IPR004154 Domain Anticodon-binding
IPR006195 Domain Aminoacyl-tRNA synthetase, class II
IPR002314 Domain Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)
IPR007214 Domain YbaK/aminoacyl-tRNA synthetase-associated domain

2 Cross Referencess


0 Found In

4 GO Annotations

GO Term Gene Name Organism
GO:0004827 IPR004500
GO:0005524 IPR004500
GO:0006433 IPR004500
GO:0005737 IPR004500

1 Parent Features

DB identifier Type Name
IPR002316 Family Proline-tRNA ligase, class IIa

1 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
Araha.11756s0442.1.p PAC:28856239 Arabidopsis halleri 572  

6 Publications

First Author Title Year Journal Volume Pages PubMed ID