Protein Domain : IPR004522

Type:  Family Name:  Asparagine-tRNA ligase
Description:  Asparagine tRNA ligase () is an alpha2 dimer that belongs to class IIb.There is a striking similarity between asparagine-tRNA ligases and archaeal/eukaryotic type aspartyl-tRNA ligases () and a striking divergence of bacterial type aspartyl-tRNA ligases (). This family, AsnS, represents asparagine-tRNA ligases from the three domains of life. Some species lack this enzyme and charge tRNA(asn) by misacylation with Asp, followed by transamidation of Asp to Asn.The aminoacyl-tRNA synthetase (also known as aminoacyl-tRNA ligase) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction. These proteins differ widely in size and oligomeric state, and have limited sequence homology []. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric []. Class II aminoacyl-tRNA synthetases share an anti-parallel beta-sheet fold flanked by alpha-helices [], and are mostly dimeric or multimeric, containing at least three conserved regions [, , ]. However, tRNA binding involves an alpha-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan and valine belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, lysine, phenylalanine, proline, serine, and threonine belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c. Short Name:  Asn-tRNA-ligase

0 Child Features

3 Contains

DB identifier Type Name
IPR012340 Domain Nucleic acid-binding, OB-fold
IPR006195 Domain Aminoacyl-tRNA synthetase, class II
IPR004364 Domain Aminoacyl-tRNA synthetase, class II (D/K/N)

4 Cross Referencess


0 Found In

5 GO Annotations

GO Term Gene Name Organism
GO:0000166 IPR004522
GO:0004816 IPR004522
GO:0005524 IPR004522
GO:0006421 IPR004522
GO:0005737 IPR004522

1 Parent Features

DB identifier Type Name
IPR002312 Family Aspartyl/Asparaginyl-tRNA synthetase, class IIb

416 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
92995 D8RG18 PAC:15422242 Selaginella moellendorffii 563  
418417 D8S5M5 PAC:15406633 Selaginella moellendorffii 531  
evm.model.supercontig_29.159 PAC:16416187 Carica papaya 375  
evm.model.supercontig_78.58 PAC:16426236 Carica papaya 572  
29912.m005417 B9RKD1 PAC:16813294 Ricinus communis 364  
29916.m000518 B9SSE8 PAC:16813512 Ricinus communis 187  
Cucsa.387780.1 A0A0A0K8G1 PAC:16982763 Cucumis sativus 613  
Cucsa.387780.2 PAC:16982764 Cucumis sativus 508  
Cucsa.289040.1 A0A0A0LYN5 PAC:16975160 Cucumis sativus 572  
Cucsa.320930.1 A0A0A0L5I1 PAC:16977417 Cucumis sativus 562  
Cucsa.320930.2 PAC:16977418 Cucumis sativus 525  
Cucsa.320930.3 PAC:16977419 Cucumis sativus 422  
GSVIVT01013821001 PAC:17824986 Vitis vinifera 496  
GSVIVT01015254001 PAC:17826044 Vitis vinifera 517  
GSVIVT01004706001 D7U3N1 PAC:17819103 Vitis vinifera 584  
orange1.1g011197m A0A067F5M9 PAC:18094339 Citrus sinensis 491  
orange1.1g011364m A0A067FE00 PAC:18094340 Citrus sinensis 487  
orange1.1g012426m A0A067FDA7 PAC:18094341 Citrus sinensis 464  
orange1.1g012529m A0A067F217 PAC:18094342 Citrus sinensis 461  
orange1.1g012612m A0A067F262 PAC:18094343 Citrus sinensis 460  
orange1.1g008405m A0A067EL65 PAC:18104916 Citrus sinensis 567  
orange1.1g010544m A0A067EPW0 PAC:18104917 Citrus sinensis 507  
AT1G70980.1 Q9SSK1 PAC:19653252 Arabidopsis thaliana 571  
AT4G17300.1 O48593 PAC:19644977 Arabidopsis thaliana 567  
AT3G07420.1 Q9SW95 PAC:19662031 Arabidopsis thaliana 638  
AT5G56680.1 Q9SW96 PAC:19672341 Arabidopsis thaliana 572  
Thhalv10024799m V4MR94 PAC:20192816 Eutrema salsugineum 564  
Thhalv10020277m V4M326 PAC:20181723 Eutrema salsugineum 639  
Thhalv10000517m V4NJ62 PAC:20208846 Eutrema salsugineum 582  
Thhalv10013096m V4KXL7 PAC:20204939 Eutrema salsugineum 575  

6 Publications

First Author Title Year Journal Volume Pages PubMed ID