Protein Domain : IPR004529

Type:  Family Name:  Phenylalanyl-tRNA synthetase, class IIc, alpha subunit
Description:  The aminoacyl-tRNA synthetase (also known as aminoacyl-tRNA ligase) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction. These proteins differ widely in size and oligomeric state, and have limited sequence homology []. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric []. Class II aminoacyl-tRNA synthetases share an anti-parallel beta-sheet fold flanked by alpha-helices [], and are mostly dimeric or multimeric, containing at least three conserved regions [, , ]. However, tRNA binding involves an alpha-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan and valine belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, lysine, phenylalanine, proline, serine, and threonine belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c.Phenylalanyl-tRNA synthetase () is an alpha2/beta2 tetramer composed of 2 subunits that belongs to class IIc. In eubacteria, a small subunit (pheS gene) can be designated as beta (E. coli) or alpha subunit (nomenclature adopted in InterPro). Reciprocally the large subunit(pheT gene) can be designated as alpha (E. coli) or beta (see and ). In all other kingdoms the two subunits have equivalent length in eukaryota, and can be identified by specific signatures. The enzyme from Thermus thermophilushas an alpha 2 beta 2 type quaternary structure and is one of the most complicated members of the synthetase family. Identification of phenylalanyl-tRNA synthetase as a member of class II aaRSs was based only on sequence alignment of the small alpha-subunit with other synthetases [].This family describes the alpha subunit, which shows some similarity to class II aminoacyl-tRNA ligases. Mitochondrial phenylalanyl-tRNA synthetase is a single polypeptide chain, active as a monomer, and similar to this chain rather than to the beta chain, but excluded from this family. Short Name:  Phe-tRNA-synth_IIc_asu

2 Child Features

DB identifier Type Name
IPR022917 Family Phenylalanine-tRNA ligase alpha subunit, bacterial/archaeal
IPR022911 Family Phenylalanine-tRNA ligase alpha chain 1, bacterial

3 Contains

DB identifier Type Name
IPR002319 Domain Phenylalanyl-tRNA synthetase
IPR006195 Domain Aminoacyl-tRNA synthetase, class II
IPR004188 Domain Phenylalanine-tRNA ligase, class II, N-terminal

1 Cross References


0 Found In

5 GO Annotations

GO Term Gene Name Organism
GO:0000166 IPR004529
GO:0004826 IPR004529
GO:0005524 IPR004529
GO:0006432 IPR004529
GO:0005737 IPR004529

0 Parent Features

189 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
231153 D8RBE9 PAC:15414604 Selaginella moellendorffii 499  
evm.model.supercontig_62.152 PAC:16423953 Carica papaya 488  
30190.m010899 B9RBF3 PAC:16823263 Ricinus communis 492  
Cucsa.321590.10 PAC:16977530 Cucumis sativus 440  
Cucsa.321590.1 A0A0A0L425 PAC:16977529 Cucumis sativus 492  
Cucsa.321590.3 A0A0A0L425 PAC:16977532 Cucumis sativus 492  
GSVIVT01031967001 D7TQ16 PAC:17838031 Vitis vinifera 488  
AT4G39280.2 F4JVC5 PAC:19648220 Arabidopsis thaliana 485  
AT4G39280.1 Q9T034 PAC:19648219 Arabidopsis thaliana 485  
Thhalv10007412m V4MVR1 PAC:20188326 Eutrema salsugineum 506  
Ciclev10028310m V4SHJ3 PAC:20813622 Citrus clementina 488  
Ciclev10028669m V4S7T7 PAC:20813624 Citrus clementina 375  
Ciclev10028421m V4SCC5 PAC:20813623 Citrus clementina 453  
Carubv10023099m R0HP34 PAC:20901248 Capsella rubella 486  
Carubv10019105m R0HP48 PAC:20886714 Capsella rubella 459  
Carubv10004690m R0GW57 PAC:20895712 Capsella rubella 485  
MDP0000888080 PAC:22674796 Malus domestica 258  
MDP0000945111 PAC:22639194 Malus domestica 268  
Lus10032123 PAC:23161179 Linum usitatissimum 528  
Lus10014563 PAC:23170921 Linum usitatissimum 513  
Potri.004G155000.1 B9H0R2 PAC:26990023 Populus trichocarpa 492  
Potri.009G116100.2 PAC:26986157 Populus trichocarpa 484  
Potri.009G116100.3 PAC:26986156 Populus trichocarpa 488  
Potri.009G116100.1 PAC:26986155 Populus trichocarpa 492  
Gorai.012G155500.1 A0A0D2S3P2 PAC:26827846 Gossypium raimondii 489  
Gorai.012G155500.2 A0A0D2V5S4 PAC:26827847 Gossypium raimondii 488  
mrna14842.1-v1.0-hybrid PAC:27263215 Fragaria vesca 616  
16778 I0YV74 PAC:27385824 Coccomyxa subellipsoidea C-169 494  
70771 C1MNK4 PAC:27347532 Micromonas pusilla CCMP1545 511  
64197 C1EHE6 PAC:27403647 Micromonas sp. RCC299 507  

7 Publications

First Author Title Year Journal Volume Pages PubMed ID