Protein Domain : IPR004499

Type:  Family Name:  Proline-tRNA ligase, class IIa, archaeal-type
Description:  Proline-tRNA ligase (also known as Prolyl-tRNA synthetase) is a class II tRNA ligase and is recognised by , which recognises tRNA ligases for Gly, His, Ser, and Pro. The proline-tRNA ligases are divided into two widely divergent families. This family includes the archaeal enzyme, the Pro-specific domain of a human multifunctional tRNA ligase, and the enzyme from the spirochete Borrelia burgdorferi(Lyme desease spirochete). The other family, , includes enzymes from Escherichia coli, Bacillus subtilis, Synechocystis sp.(strain PCC 6308), and one of the two proline-tRNA ligases of Saccharomyces cerevisiae(Baker's yeast).The aminoacyl-tRNA synthetase (also known as aminoacyl-tRNA ligase) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction. These proteins differ widely in size and oligomeric state, and have limited sequence homology []. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric []. Class II aminoacyl-tRNA synthetases share an anti-parallel beta-sheet fold flanked by alpha-helices [], and are mostly dimeric or multimeric, containing at least three conserved regions [, , ]. However, tRNA binding involves an alpha-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan and valine belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, lysine, phenylalanine, proline, serine, and threonine belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c. Short Name:  Pro-tRNA-ligase_IIa_arc-type

0 Child Features

5 Contains

DB identifier Type Name
IPR004154 Domain Anticodon-binding
IPR006195 Domain Aminoacyl-tRNA synthetase, class II
IPR002314 Domain Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)
IPR016061 Domain Proline-tRNA ligase, class II, C-terminal
IPR015264 Domain Prolyl-tRNA synthetase, class II, C-terminal, archaeal-type

3 Cross Referencess


0 Found In

4 GO Annotations

GO Term Gene Name Organism
GO:0004827 IPR004499
GO:0005524 IPR004499
GO:0006433 IPR004499
GO:0005737 IPR004499

1 Parent Features

DB identifier Type Name
IPR002316 Family Proline-tRNA ligase, class IIa

360 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
94171 D8RIK0 PAC:15421940 Selaginella moellendorffii 528  
74713 D8QP68 PAC:15412093 Selaginella moellendorffii 495  
evm.model.supercontig_285.2 PAC:16416068 Carica papaya 500  
evm.model.supercontig_107.92 PAC:16405401 Carica papaya 160  
evm.model.supercontig_107.96 PAC:16405405 Carica papaya 544  
30076.m004552 B9RN41 PAC:16817164 Ricinus communis 501  
30169.m006591 B9RIU3 PAC:16821390 Ricinus communis 548  
Cucsa.049590.1 PAC:16954009 Cucumis sativus 555  
Cucsa.094750.1 A0A0A0KTZ1 PAC:16957781 Cucumis sativus 511  
Cucsa.094750.2 A0A0A0KTZ1 PAC:16957782 Cucumis sativus 511  
GSVIVT01028877001 E0CUX2 PAC:17835876 Vitis vinifera 448  
GSVIVT01028250001 D7U2U2 PAC:17835444 Vitis vinifera 516  
orange1.1g010398m A0A067H4L9 PAC:18137303 Citrus sinensis 511  
orange1.1g010413m A0A067H4L9 PAC:18137304 Citrus sinensis 511  
orange1.1g010430m A0A067H4L9 PAC:18137305 Citrus sinensis 511  
orange1.1g047998m A0A067ENV9 PAC:18125422 Citrus sinensis 558  
AT5G52520.1 Q9FYR6 PAC:19667286 Arabidopsis thaliana 543  
AT3G62120.1 Q9M1R2 PAC:19664205 Arabidopsis thaliana 530  
AT3G62120.2 Q9M1R2 PAC:19664206 Arabidopsis thaliana 530  
Thhalv10005900m V4L0R3 PAC:20190206 Eutrema salsugineum 512  
Thhalv10013193m V4LTQ7 PAC:20204648 Eutrema salsugineum 541  
Thhalv10011470m V4JYN4 PAC:20184668 Eutrema salsugineum 451  
Ciclev10031253m V4VAS8 PAC:20803279 Citrus clementina 517  
Ciclev10019765m V4U6Q8 PAC:20812061 Citrus clementina 511  
Ciclev10004659m V4U7L4 PAC:20789919 Citrus clementina 558  
Carubv10017007m R0HIX5 PAC:20888621 Capsella rubella 527  
Carubv10018634m R0FRR9 PAC:20887043 Capsella rubella 522  
Carubv10026191m R0GPR7 PAC:20913659 Capsella rubella 543  
Carubv10027520m R0GCE9 PAC:20914204 Capsella rubella 495  
MDP0000291077 PAC:22664713 Malus domestica 509  

6 Publications

First Author Title Year Journal Volume Pages PubMed ID