Protein Domain : IPR002319

Type:  Domain Name:  Phenylalanyl-tRNA synthetase
Description:  The aminoacyl-tRNA synthetase (also known as aminoacyl-tRNA ligase) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction. These proteins differ widely in size and oligomeric state, and have limited sequence homology []. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric []. Class II aminoacyl-tRNA synthetases share an anti-parallel beta-sheet fold flanked by alpha-helices [], and are mostly dimeric or multimeric, containing at least three conserved regions [, , ]. However, tRNA binding involves an alpha-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan and valine belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, lysine, phenylalanine, proline, serine, and threonine belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c.Phenylalanyl-tRNA synthetase () is an alpha2/beta2 tetramer composed of 2 subunits that belongs to class IIc. In eubacteria, a small subunit (pheS gene) can be designated as beta (E. coli) or alpha subunit (nomenclature adopted in InterPro). Reciprocally the large subunit(pheT gene) can be designated as alpha (E. coli) or beta (see and ). In all other kingdoms the two subunits have equivalent length in eukaryota, and can be identified by specific signatures. The enzyme from Thermus thermophilushas an alpha 2 beta 2 type quaternary structure and is one of the most complicated members of the synthetase family. Identification of phenylalanyl-tRNA synthetase as a member of class II aaRSs was based only on sequence alignment of the small alpha-subunit with other synthetases []. Short Name:  Phenylalanyl-tRNA_Synthase

0 Child Features

2 Contains

DB identifier Type Name
IPR006195 Domain Aminoacyl-tRNA synthetase, class II
IPR004188 Domain Phenylalanine-tRNA ligase, class II, N-terminal

1 Cross References


3 Found Ins

DB identifier Type Name
IPR004529 Family Phenylalanyl-tRNA synthetase, class IIc, alpha subunit
IPR004530 Family Phenylalanyl-tRNA synthetase, class IIc, mitochondrial
IPR005246 Family O-phosphoseryl-tRNA(Cys) ligase

5 GO Annotations

GO Term Gene Name Organism
GO:0000049 IPR002319
GO:0004812 IPR002319
GO:0005524 IPR002319
GO:0043039 IPR002319
GO:0005737 IPR002319

0 Parent Features

501 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
165421 PAC:15413457 Selaginella moellendorffii 470  
231153 D8RBE9 PAC:15414604 Selaginella moellendorffii 499  
evm.model.supercontig_62.152 PAC:16423953 Carica papaya 488  
evm.model.supercontig_84.15 PAC:16427294 Carica papaya 426  
29620.m000542 B9SSI2 PAC:16803795 Ricinus communis 438  
35700.m000027 B9TFK2 PAC:16826706 Ricinus communis 58  
30190.m010899 B9RBF3 PAC:16823263 Ricinus communis 492  
Cucsa.048740.1 A0A0A0KJ29 PAC:16953911 Cucumis sativus 431  
Cucsa.048740.5 A0A0A0KJ29 PAC:16953915 Cucumis sativus 431  
Cucsa.048740.6 A0A0A0KJ29 PAC:16953916 Cucumis sativus 431  
Cucsa.048740.8 PAC:16953918 Cucumis sativus 314  
Cucsa.321590.10 PAC:16977530 Cucumis sativus 440  
Cucsa.321590.1 A0A0A0L425 PAC:16977529 Cucumis sativus 492  
Cucsa.321590.3 A0A0A0L425 PAC:16977532 Cucumis sativus 492  
GSVIVT01010923001 D7UBM0 PAC:17822937 Vitis vinifera 427  
GSVIVT01020347001 D7SWT1 PAC:17829756 Vitis vinifera 314  
GSVIVT01020319001 D7SWR6 PAC:17829740 Vitis vinifera 191  
GSVIVT01020310001 PAC:17829734 Vitis vinifera 75  
GSVIVT01020335001 D7SWS4 PAC:17829749 Vitis vinifera 281  
GSVIVT01020339001 PAC:17829751 Vitis vinifera 424  
GSVIVT01031967001 D7TQ16 PAC:17838031 Vitis vinifera 488  
orange1.1g014218m A0A067EHX6 PAC:18115210 Citrus sinensis 428  
orange1.1g018851m A0A067ECZ3 PAC:18134487 Citrus sinensis 349  
AT4G39280.2 F4JVC5 PAC:19648220 Arabidopsis thaliana 485  
AT3G58140.1 Q94K73 PAC:19662875 Arabidopsis thaliana 429  
AT4G39280.1 Q9T034 PAC:19648219 Arabidopsis thaliana 485  
Thhalv10005992m V4LWI6 PAC:20190444 Eutrema salsugineum 430  
Thhalv10007412m V4MVR1 PAC:20188326 Eutrema salsugineum 506  
Ciclev10028310m V4SHJ3 PAC:20813622 Citrus clementina 488  
Ciclev10028669m V4S7T7 PAC:20813624 Citrus clementina 375  

7 Publications

First Author Title Year Journal Volume Pages PubMed ID