Protein Domain : IPR004530

Type:  Family Name:  Phenylalanyl-tRNA synthetase, class IIc, mitochondrial
Description:  The aminoacyl-tRNA synthetase (also known as aminoacyl-tRNA ligase) catalyse the attachment of an amino acid to its cognate transfer RNA molecule in a highly specific two-step reaction. These proteins differ widely in size and oligomeric state, and have limited sequence homology []. The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. Class I aminoacyl-tRNA synthetases contain a characteristic Rossman fold catalytic domain and are mostly monomeric []. Class II aminoacyl-tRNA synthetases share an anti-parallel beta-sheet fold flanked by alpha-helices [], and are mostly dimeric or multimeric, containing at least three conserved regions [, , ]. However, tRNA binding involves an alpha-helical structure that is conserved between class I and class II synthetases. In reactions catalysed by the class I aminoacyl-tRNA synthetases, the aminoacyl group is coupled to the 2'-hydroxyl of the tRNA, while, in class II reactions, the 3'-hydroxyl site is preferred. The synthetases specific for arginine, cysteine, glutamic acid, glutamine, isoleucine, leucine, methionine, tyrosine, tryptophan and valine belong to class I synthetases. The synthetases specific for alanine, asparagine, aspartic acid, glycine, histidine, lysine, phenylalanine, proline, serine, and threonine belong to class-II synthetases. Based on their mode of binding to the tRNA acceptor stem, both classes of tRNA synthetases have been subdivided into three subclasses, designated 1a, 1b, 1c and 2a, 2b, 2c.Phenylalanyl-tRNA synthetase () is an alpha2/beta2 tetramer composed of 2 subunits that belongs to class IIc. In eubacteria, a small subunit (pheS gene) can be designated as beta (E. coli) or alpha subunit (nomenclature adopted in InterPro). Reciprocally the large subunit(pheT gene) can be designated as alpha (E. coli) or beta (see and ). In all other kingdoms the two subunits have equivalent length in eukaryota, and can be identified by specific signatures. The enzyme from Thermus thermophilushas an alpha 2 beta 2 type quaternary structure and is one of the most complicated members of the synthetase family. Identification of phenylalanyl-tRNA synthetase as a member of class II aaRSs was based only on sequence alignment of the small alpha-subunit with other synthetases [].This family describes the mitochondrial phenylalanyl-tRNA synthetases. Unlike all other known phenylalanyl-tRNA synthetases, the mitochondrial form demonstrated from yeast is monomeric. It is similar to but longer than the alpha subunit (PheS) of the alpha 2 beta 2 form found in bacteria, Archaea, and eukaryotes, and shares the characteristic motifs of class II aminoacyl-tRNA ligases. Short Name:  Phe-tRNA-synth_IIc_mito

0 Child Features

3 Contains

DB identifier Type Name
IPR005121 Domain Phenylalanine-tRNA ligase, beta subunit, ferrodoxin-fold anticodon-binding
IPR002319 Domain Phenylalanyl-tRNA synthetase
IPR006195 Domain Aminoacyl-tRNA synthetase, class II

1 Cross References


0 Found In

5 GO Annotations

GO Term Gene Name Organism
GO:0000166 IPR004530
GO:0004826 IPR004530
GO:0005524 IPR004530
GO:0006432 IPR004530
GO:0005737 IPR004530

0 Parent Features

90 Proteins

DB identifier UniProt Accession Secondary Identifier Organism Name Length
Cucsa.048740.1 A0A0A0KJ29 PAC:16953911 Cucumis sativus 431  
Cucsa.048740.5 A0A0A0KJ29 PAC:16953915 Cucumis sativus 431  
Cucsa.048740.6 A0A0A0KJ29 PAC:16953916 Cucumis sativus 431  
Cucsa.048740.8 PAC:16953918 Cucumis sativus 314  
GSVIVT01010923001 D7UBM0 PAC:17822937 Vitis vinifera 427  
orange1.1g014218m A0A067EHX6 PAC:18115210 Citrus sinensis 428  
AT3G58140.1 Q94K73 PAC:19662875 Arabidopsis thaliana 429  
Thhalv10005992m V4LWI6 PAC:20190444 Eutrema salsugineum 430  
Carubv10017303m R0HG51 PAC:20887178 Capsella rubella 430  
Potri.013G105200.1 PAC:26993442 Populus trichocarpa 480  
Potri.013G105200.2 PAC:26993443 Populus trichocarpa 473  
Gorai.011G155000.1 A0A0D2T8Q6 PAC:26810971 Gossypium raimondii 428  
52075 I0Z8Z3 PAC:27388998 Coccomyxa subellipsoidea C-169 387  
213234 PAC:27340662 Micromonas pusilla CCMP1545 424  
61175 C1EBS7 PAC:27401565 Micromonas sp. RCC299 415  
30154 A4RSR3 PAC:27414581 Ostreococcus lucimarinus 416  
119609 A4RSR3 PAC:27414566 Ostreococcus lucimarinus 416  
Pavir.Cb00909.1.p PAC:30269295 Panicum virgatum 452  
Pavir.J01957.1.p PAC:30195553 Panicum virgatum 454  
Pavir.J01957.2.p PAC:30195552 Panicum virgatum 454  
Glyma.18G291800.1.p K7MVE7 PAC:30555866 Glycine max 431  
Glyma.18G291800.2.p K7MVE7 PAC:30555865 Glycine max 431  
Glyma.18G291800.3.p A0A0R0FGB9 PAC:30555867 Glycine max 373  
Glyma.02G091300.2.p I1JDN2 PAC:30507643 Glycine max 431  
Glyma.02G091300.3.p I1JDN2 PAC:30507644 Glycine max 431  
Glyma.02G091300.4.p I1JDN2 PAC:30507641 Glycine max 431  
Glyma.02G091300.1.p I1JDN2 PAC:30507642 Glycine max 431  
Brara.G01781.1.p PAC:30632708 Brassica rapa FPsc 430  
Brara.I04015.1.p PAC:30643108 Brassica rapa FPsc 430  
Cre02.g079600.t1.2 PAC:30785296 Chlamydomonas reinhardtii 434  

7 Publications

First Author Title Year Journal Volume Pages PubMed ID